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1.13.99.1: inositol oxygenase

This is an abbreviated version!
For detailed information about inositol oxygenase, go to the full flat file.

Word Map on EC 1.13.99.1

Reaction

myo-inositol
+
O2
=
D-glucuronate
 +
H2O

Synonyms

amyoinositol oxygenase, AtMIOX, EC 1.13.1.11, EC 1.99.2.6, GsMIOX1a, Inositol oxygenase, inositol oxygenase 1, InOx, Kidney-specific protein 32, meso-Inositol oxygenase, MIOX, MIOX1, MIOX2, MIOX4, MIOX5, mMIOX, MOO, Myo-inositol oxygenase, OsMIOX, Oxygenase, inositol, ppMIOX, Renal-specific oxidoreductase, renal-specific oxidoreductase/myo-inositol oxygenase, RSOR/MIOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.99 Miscellaneous
                1.13.99.1 inositol oxygenase

Cloned

Cloned on EC 1.13.99.1 - inositol oxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli in the presence of the iron chelator, 1,10-phenanthroline to create iron-free MIOX
-
expressed in Escherichia coli strain BW25113
expressed in Escherichia coli strain EA31 and Saccharomyces cerevisiae strain SG
expressed in LLC-PK1 cells
expression in Agrobacterium tumefaciens
expression in epithelial cell line LLC-PK1
expression in Escherichia coli
gene AtMIOX1, phylogenetic tree, semi-quantitative reverse transcriptase enzyme expression analysis
gene GsMIOX1a, genetic structure, phylogenetic tree, ectopic expression under control of the constitutive CaMV35S promoter in Arabidopsis thaliana leads to enhanced tolerance to alkaline stress, while expression of enzyme mutant atmiox1 mutant decreases alkaline stress tolerance in Arabidopsis thaliana ecotype Columbia, quantitative real-time PCR enzyme expression analysis. The expression levels of some alkaline stress-responsive and inducible marker genes, including H+-Ppase, NADP-ME, KIN1 and RD29B, are also up-regulated in GsMIOX1a overexpression lines compared with the wild-type and atmiox1 mutant expressing plants
gene MIOX, kidney-specific expression, recombinant expression of GST-tagged enzyme in HEK-293 cells, cleavage of the GST tag by thrombin
gene MIOX, quantitative enzyme expression analysis
gene MIOX, quantitative real-time PCR enzyme expression analysis
gene MIOX, quantitative real-time PCR enzyme expression analysis, overexpression of MIOX in LLC-PK1 cells boosts the generation of H2O2
gene miox, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein
gene MIOX1, transient recombinant overexpression of GFP-tagged OsMIOX, co-localizing with the endoplasmic reticulum marker, in Nicotiana benthamiana protoplasts, quantitative real-Time PCR enzyme expression analysis, recombinant expression of GST-tagged OsMIOX1 with MBP-tagged PeaT1 in Saccharomyces cerevisiae in the two-hybrid-system
gene mMIOX, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440, recombinant coexpression of the enzyme in Escherichia coli with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440
genotyping of MIOX in Caucasian type I diabetes mellitus patients, overview
-
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX1, quantitative real-time PCR enzyme expression analysis
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX2, quantitative real-time PCR enzyme expression analysis
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX4, quantitative real-time PCR enzyme expression analysis
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX5, quantitative real-time PCR enzyme expression analysis
open reading frame of 849 base pairs
transfection into NRK-52E cells
-