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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
+
ATP
=
firefly oxyluciferin
+
CO2
+
AMP
+
diphosphate
+
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

pH Stability

pH Stability on EC 1.13.12.7 - firefly luciferase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
all spectra undergo a red-shift when cells are assayed under acidic conditions, whereas a blue-shift is observed at pH 8.0
676934
8 - 10
recombinant circular LUC is stable at a pH range of 8.0-10.0, maintaining nearly 100% activity after incubation in various pH buffers for 30 min. Recombinant wild-type LUC maintains only 80% of its original activity after incubating at pH 10.0 for 30 min
746213