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1.13.12.5: Renilla-type luciferase

This is an abbreviated version!
For detailed information about Renilla-type luciferase, go to the full flat file.

Word Map on EC 1.13.12.5

Reaction

coelenterazine h
+
O2
=
coelenterazine h dioxetanone

Synonyms

19kOLase, aequorin, aequorin-1, BFP-aq, blue fluorescent protein from the calcium-binding photoprotein aequorin, Caussia princeps luciferase, clytin, extGLuc, extRLuc, firefly luciferase, fluc, Gaussia luciferase, Gaussia luciferase enzyme, Gaussia princeps luciferase, Gaussia-luciferase, GFP-aq, GLase, GLuc, Luc, luciferase, luciferase (Renilla luciferin), m-Rluc8, membrane-anchored RLUc, Oplophorus luciferase, PI-Rluc, R-Luc, Renilla luciferase, Renilla luciferase-547, Renilla luciferin 2-monooxygenase, Renilla muelleri luciferase, Renilla reniformis luciferase, Renilla-luciferase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase, RLase, RLase-547, RLuc, RLuc8, short peptide-inserted-Renilla luciferase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.5 Renilla-type luciferase

Purification

Purification on EC 1.13.12.5 - Renilla-type luciferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
Hi-Trap column
Renilla sp.
-
natively folded GLuc is obtained by bacterial expression and efficient refolding using a solubility enhancement petide tag
recombinant His-tagged engineered enzyme C-SRLuc8 in Escherichia coli by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography to over 95% purity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant secreted enzyme
recombinant soluble His6-tagged enzyme FcUni-RLuc from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
souble and insoluble fraction by nickel-chelate column
-
using Ni-chelate column