1.13.12.13: Oplophorus-luciferin 2-monooxygenase

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For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

19kOLase, CXXC-Oluc, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

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Results	in table
1	AA Sequence
3	Application
8	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
21	Inhibitors
18	Ki Value [mM]
4	KM Value [mM]
4	Localization
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organism
15	PDB ID
4	pH Optimum
29	Protein Variants
6	Purification (Commentary)
1	Reaction
14	Reference
1	Renatured (Commentary)
1	Source Tissue
4	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
5	Subunits
18	Synonyms
1	Systematic Name
2	Temperature Stability [°C]

Purification

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Purification on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Oplophorus gracilirostris

-

654469

form inclusion bodies of recombinant Escherichia coli, solubilization with 6 M urea

Oplophorus gracilirostris

-

689868

recombinant His-tagged catalytic subunit mutant nanoKAZ from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis, wild-typeKAZ is purified from inclusion bodies using 6 M urea-nickel chelate affinity column chromatography

Oplophorus gracilirostris

Q9GV45; Q9GV46

744231

recombinant His-tagged catalytic wild-type and mutant enzymes and catalytic subunits from Escherichia coli by nickel affinity chromatgraphy

Oplophorus gracilirostris

Q9GV45; Q9GV46

744220

using Ni-NTA chromatography

Oplophorus gracilirostris

-

726882

using nickel-chelate affinity column

Oplophorus gracilirostris

-

728741