1.13.12.13: Oplophorus-luciferin 2-monooxygenase
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For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.
Word Map on EC 1.13.12.13
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1.13.12.13
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bioluminescence
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luminescence
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firefly
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luciferases
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gracilirostris
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nanobit
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furimazine
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lgbit
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renilla
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brightest
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coelenterazine
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gaussia
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molecular biology
- 1.13.12.13
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bioluminescence
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luminescence
- firefly
- luciferases
- gracilirostris
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nanobit
- furimazine
-
lgbit
- renilla
-
brightest
- coelenterazine
- gaussia
- molecular biology
Reaction
Synonyms
19kOLase, CXXC-Oluc, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase
ECTree
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Application
Application on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase
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molecular biology
useful reporter protein in various assay systems including reporter assays and immunoassays
molecular biology
Oplophorus sp.
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a multicolor BRET assay for the quantification of global DNA methylation is developed using CXXC-fused Oplophorus luciferase (CXXC-Oluc), a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc), BOBO-1, and BOBO-3. CXXC-Oluc recognized unmethylated CpG sites on genomic DNA to excite BOBO-1 DNA intercalating dye, whereas MBD-Fluc recognized methylated CpG sites on genomic DNA to excite BOBO-3 DNA intercalating dye. The emission intensities of BOBO-1 and BOBO-3 are simultaneously detected and depended on the unmethylated and methylated CpG contents of the genomic DNA. There is a significant negative correlation between the emission intensities of BOBO-1 and BOBO-3. Therefore, the global DNA methylation level can be quantified with this multicolor BRET assay using a single tube
molecular biology
Q9GV45; Q9GV46
bioluminescence imaging is a powerful, broadly utilized method for non-invasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. The use of a luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, is described in cell-based assays bioluminescence imaging of tumor-bearing mice. NanoLuc is combined with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session