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1.13.11.63: beta-carotene 15,15'-dioxygenase

This is an abbreviated version!
For detailed information about beta-carotene 15,15'-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.63

Reaction

beta-carotene
+
O2
= 2 all-trans retinal

Synonyms

BC-15,15'-oxygenase, BCM, BCMO, Bcmo1, Bcmo2, BCO, BCO1, BCO2, Bcox, beta,beta-carotene 15,15'-mono-oxygenase 1, beta,beta-carotene 15,15'-monooxygenase, beta,beta-carotene 15,15'-monooxygenase 1, beta,beta-carotene 15,15'-monooxygenase-1, beta,beta-carotene 15,15-monooxygenase 1, beta,beta-carotene-15,15'-monooxygenase, beta,beta-carotene-15,15'-oxygenase, beta-carotene 15,15' oxygenase, beta-carotene 15,15'-dioxygenase, beta-carotene 15,15'-monooxygenase, beta-carotene 15,15'-monooxygenase 1, beta-carotene 15,15'-oxygenase, beta-carotene 15,15-monooxygenase, beta-carotene dioxygenase 1, beta-carotene oxygenase 2, beta-carotene-15,15'-dioxygenase, beta-carotene-15,15'-monooxygenase, beta-carotene-15,15'-oxygenase, beta-carotene-15,15'-oxygenase-1, beta-carotene-15,15'-oxygenase-2, beta-CD, betaCDIOX, betaCO, BLH, brp/blh-family beta-carotene monooxygenase, brpA, carotenoid oxygenase, Ci-BCO, CMO1, EC 1.13.11.21, EC 1.14.99.36, NinaB

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.63 beta-carotene 15,15'-dioxygenase

Purification

Purification on EC 1.13.11.63 - beta-carotene 15,15'-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
-
by His-Trap HP affinity and Resource Q ion exchange chromatography
native and recombinant protein
native enzyme partially from Stellate cell cytosol
Ni-NTA agarose resin column chromatography
partial
recombinant BCO1 with a C-terminal polyhistidine tag from Escherichia coli strain BL21-Gold(DE3) by cobalt affinity chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli by affinity and anion exchange chromatography
recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags from Spodoptera frugiperda Sf9 cells by Co2+ affinity chromatography and gel filtration in absence of detergent
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
using a His-Trap HP affinity chromatography column
using a HiTrap Chelating HP column