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1.13.11.54: acireductone dioxygenase [iron(II)-requiring]

This is an abbreviated version!
For detailed information about acireductone dioxygenase [iron(II)-requiring], go to the full flat file.

Word Map on EC 1.13.11.54

Reaction

1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one
+
O2
=
4-(methylsulfanyl)-2-oxobutanoate
 +
formate

Synonyms

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, aci-reductone dioxygenase, acireductone dioxygenase, acireductone dioxygenase 1, ADI1, ARD, ARD', ARD1, ARD4, ARDp, Fe(II)-bound acireductone dioxygenase, Fe-ARD, MTCBP-1, MtnD, OsARD1, Ymr009p

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.54 acireductone dioxygenase [iron(II)-requiring]

Engineering

Engineering on EC 1.13.11.54 - acireductone dioxygenase [iron(II)-requiring]

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E94A
-
no activity
D101A
about 60% of wild-type activity
E100A
about 2% of wild-type activity. E100 is not essential for metal binding
E102A
no catalytic activity
E95A
about 4% of wild-type activity
H140A
no catalytic activity
H140F
no catalytic activity
H96A
no catalytic activity
H98A
no catalytic activity
H98S
no catalytic activity. Mutant exhibits little affinity for either Ni2+ or Fe2+, indicating that His 98 is likely involved in binding both metals
D101A
-
about 60% of wild-type activity
-
E100A
-
about 2% of wild-type activity. E100 is not essential for metal binding
-
H140A
-
no catalytic activity
-
H96A
-
no catalytic activity
-
additional information