1.13.11.50: acetylacetone-cleaving enzyme

This is an abbreviated version!
For detailed information about acetylacetone-cleaving enzyme, go to the full flat file.

Reaction

Synonyms

acetylacetone dioxygenase, acetylacetone-cleaving enzyme, b-diketone dioxygenase, beta-diketone dioxygenase, cupin-type dioxygenase, diketone cleaving dioxygenase, diketone cleaving enzyme, diketone dioxygenase, diketone-cleaving dioxygenase, diketone-cleaving enzyme, DKDO, Dke1, oxygenase, b-diketone di-, pentane-2,4-dione hydrolase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.50 acetylacetone-cleaving enzyme

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Results	in table
14	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
3	General Information
1	General Stability
11	Inhibitors
15	KM Value [mM]
1	Localization
22	Metals/Ions
11	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
15	Organism
4	PDB ID
4	pH Optimum
2	pH Range
25	Protein Variants
6	Purification (Commentary)
4	Reaction
5	Reaction Type
15	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
6	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
31	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 1.13.11.50 - acetylacetone-cleaving enzyme

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

1,1,1-trifluoro-2,4-pentanedione + O2

?

Acinetobacter johnsonii

-

-

674150

-

-

?

1,1,1-trifluoro-pentane-2,4-dione + O2

?

2 entries

1,1-difluoro-2,4-pentanedione + O2

?

Acinetobacter johnsonii

-

-

674150

-

-

?

1,1-difluoropentane-2,4-dione + O2

?

Acinetobacter johnsonii

-

-

725553

-

-

?

1-phenyl-1,3-butanedione + O2

?

Acinetobacter johnsonii

-

-

636328

-

-

?

1-phenylbutane-1,3-dione + O2

?

Acinetobacter johnsonii

-

-

657866

-

-

?

2,4-dioxopentanoic acid ethyl ester + O2

?

2 entries

2,4-nonadione + O2

?

Acinetobacter johnsonii

-

poor substrate

636328

-

-

?

2,4-octanedione + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

2-acetylcyclohexanone + O2

?

Acinetobacter johnsonii

-

-

636327, 657866

-

-

?

3,4-dihydroxyphenylacetate + O2

?

Acinetobacter johnsonii

Q8GNT2

-

696107

-

-

?

3,5-heptanedione + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

3-methyl-2,4-pentanedione + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

3-oxobutanone + O2

?

Acinetobacter johnsonii

-

-

636327, 657866

-

-

?

4,4-difluoro-1-phenyl-1,3-butanedione + O2

?

Acinetobacter johnsonii

-

-

674150

-

-

?

4-hydroxy-4-methyl-2-pentanone + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

5,5-dimethylhexane-2,4-dione + O2

?

Acinetobacter johnsonii

-

poor substrate

636328

-

-

?

acetylacetone + O2

acetate + 2-oxopentanal

Acinetobacter johnsonii

-

-

711258

-

-

?

acetylcyclohexanone + O2

?

Acinetobacter johnsonii

-

poor substrate

636328

-

-

?

ethylacetoacetate + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

pentane-2,4-dione + O2

acetate + 2-oxopropanal

3 entries

pentane-2,4-dione + O2

acetate + methylglyoxal

Acinetobacter johnsonii

-

-

725198, 725553

-

-

?

pentane-2,4-dione + O2

methylglyoxal + acetate

Acinetobacter johnsonii

Q8GNT2

-

696107

-

-

?

potassium oxalate + O2

?

Acinetobacter johnsonii

Q8GNT2

-

696107

-

-

?

quercetin + O2

?

Acinetobacter johnsonii

Q8GNT2

-

696107

-

-

?

additional information

?

-

8 entries

1,1,1-trifluoro-pentane-2,4-dione + O2

?

Acinetobacter johnsonii

-

-

675466

-

-

?

1,1,1-trifluoro-pentane-2,4-dione + O2

?

Acinetobacter johnsonii

Q8GNT2

-

711267

-

-

?

2,4-dioxopentanoic acid ethyl ester + O2

?

Acinetobacter johnsonii

-

-

636327

-

-

?

2,4-dioxopentanoic acid ethyl ester + O2

?

Acinetobacter johnsonii

-

poor substrate

636328

-

-

?

pentane-2,4-dione + O2

acetate + 2-oxopropanal

Acinetobacter johnsonii

-

-

636327, 657614, 657866, 671683, 674150, 675466

-

-

?

pentane-2,4-dione + O2

acetate + 2-oxopropanal

Acinetobacter johnsonii

Q8GNT2

-

711267

-

-

?

pentane-2,4-dione + O2

acetate + 2-oxopropanal

Acinetobacter johnsonii

-

i.e. acetylacetone

657866

i.e. acetate and acetone

-

?

additional information

?

-

Acinetobacter johnsonii

-

no substrate: 4-hydroxy-4-methyl-2-pentanone, 3-oxobutanoate, quercetin, catechols, 2,4`-dihydroxyacetophenone, ascorbate

636327

-

-

?

additional information

?

-

Acinetobacter johnsonii

-

the catalytic reaction proceeds via one common rate-limiting step in the clevage of all substrates accepted by the enzyme

657866

-

-

?

additional information

?

-

Acinetobacter johnsonii

Q8GNT2

residues Arg80 and Tyr70 promote O2 reduction

711267

-

-

?

additional information

?

-

Acinetobacter johnsonii

-

the enzyme is a mononuclear non-heme iron enzymes, Dke1 active site modelling, overview

711258

-

-

?

additional information

?

-

Acinetobacter johnsonii

-

acetylacetone dioxygenase catalyzes the dioxygen-dependent degradation of beta-dicarbonyl compounds

725198

-

-

?

additional information

?

-

Acinetobacter johnsonii

-

Dke1 also performs the atypical cleavage of the alpha-keto acid, 4-hydroxyphenylpyruvate, to form 4-hydroxybenzaldehyde as product instead of the homogentisate product found for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase, EC 1.13.11.27, analysis of the bonding of the alpha-keto acid, 4-hydroxyphenylpyruvate, to ferrous Dke1 using anaerobic Dke1, added ferrous ammonium sulfate, and 4-hydroxyphenylpyruvate at pH 7.0, overview

725198

-

-

?

additional information

?

-

Acinetobacter johnsonii

-

Ni(II)-complex-adducts show regioselective oxidative cleavage of the aliphatic C-C bond using dioxygen via the substrate activation mechanism without changing the oxidation state of nickel(II) as similar to the wild type enzyme

764544

-

-

-

additional information

?

-

Paraburkholderia xenovorans

-

iron(II) complexes [Fe(L)(CH3CN)3](SO3CF3)2 of tris(2-pyridyl)-based ligands exhibit very similar chemical surroundings to the active site of the enzyme and mimic its functions

764545

-

-

-