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1.13.11.5: homogentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about homogentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.5

Reaction

homogentisate
+
O2
=
4-Maleylacetoacetate

Synonyms

EC 1.13.1.5, EC 1.99.2.5, ElHDO, HgD, HGDO, HGO, HGOa, HGOb, HmgA, homogentisate 1,2 dioxigenase, homogentisate 1,2 dioxygenase, homogentisate 1,2-dioxygenase, homogentisate dioxygenase, homogentisate oxidase, homogentisate oxygenase, homogentisate phytyl-transferase, homogentisic acid 1,2-dioxygenase, homogentisic acid oxidase, homogentisic acid oxygenase, homogentisic oxygenase, homogentisicase, HPT, HTO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.5 homogentisate 1,2-dioxygenase

Cloned

Cloned on EC 1.13.11.5 - homogentisate 1,2-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
complete HGO cDNA, encoding for a 50 kDa protein, is cloned and sequenced
-
DNA and amino acid sequence determination and analysis, genotyping, polymorphism of the HGD gene in the exon 1 and intron 1
DNA and ammino acid sequence determinaation and analysis, located on chromosome 3(q23-3q21), genotyping and identification of narurally occuring mutations involved in alkaptonuria, overview
expressed in Burkholderia cepacia strain J2315
-
expressed in Escherichia coli
expressed in Phanerochaete sordida strain UV-64
-
expression of His-tagged HGO in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene HGD, DNA and amino acid sequence determination and analysis, genotyping, semi-quantitative RT-PCR analysis. Identification of nine single nucleotide polymorphisms, five in the coding region and four intronic
genes hmgA1 and hmgA2, the gene cluster hmgABC is located at C1 in strain LB400 genome, quantitative expression analysis
-
genotyping
-
HGO gene on chromosome 3 is cloned, completely sequenced and characterized, identification of its promoter region, transcriptional control of the gene
hmgA gene encoding homogentisate dioxygenase, a 448 residue polypeptide, is cloned, sequenced and characterized, gene is expressed in Escherichia coli as a fusion to glutathione S-transferase
PCR of coding region of DNA extracted from EDTA blood sample