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1.13.11.49: chlorite O2-lyase

This is an abbreviated version!
For detailed information about chlorite O2-lyase, go to the full flat file.

Word Map on EC 1.13.11.49

Reaction

chloride
+
O2
=
Chlorite

Synonyms

chlorite dismutase, CLD, Cyan7425_1434, dimutase, chlorite, HemQ, Pfam chlorite dismutase, PitA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.49 chlorite O2-lyase

Molecular Weight

Molecular Weight on EC 1.13.11.49 - chlorite O2-lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
Stutzerimonas chloritidismutans
-
SDS-PAGE, native enzyme with Superdex 200 column with 50 mM potassium phosphate buffer, pH 7.0 and 100 mM NaCl
115000
-
gel filtration
116000
-
gel filtration
140000
155000
pentamer, native mass spectrometry, native mass spectrometry, enzyme buffer-exchanged to 50 mM ammonium acetate, pH 6.8, with centrifugal filter units with 30 kDa cutoff
25000
-
4 * 25000, SDS-PAGE
28400
30000
30420
monomer without heme, native mass spectrometry, enzyme buffer-exchanged to 50 mM ammonium acetate, pH 6.8, with centrifugal filter units with 30 kDa cutoff
31000
Stutzerimonas chloritidismutans
-
4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS)
31030
monomer with heme, native mass spectrometry, enzyme buffer-exchanged to 50 mM ammonium acetate, pH 6.8, with centrifugal filter units with 30 kDa cutoff
31032
1 * 30420 without heme, 1 * 31032 with heme
32000
-
x * 32000, SDS-PAGE
54000
-
recombinant mutant W155F, gel filtration