1.13.11.45: linoleate 11-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 11-lipoxygenase, go to the full flat file.

Reaction

Synonyms

13 R -MnLOX, 13R-MnLOX, Cg-MnLOX, CspLOX2, Fo-MnLOX, linoleate dioxygenase, lipoxygenase 2, LOX-1, manganese lipoxygenase, manganese LOX, MGG_08499, Mn-LO, Mn-LOX, MnLOX, Mo-MnLOX

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.45 linoleate 11-lipoxygenase

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Results	in table
343	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
8	General Information
1	General Stability
9	Inhibitors
17	KM Value [mM]
2	Localization
24	Metals/Ions
3	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
17	Organism
1	Pathway
2	PDB ID
10	pH Optimum
5	pH Range
3	Posttranslational Modification
38	Protein Variants
8	Purification (Commentary)
5	Reaction
4	Reaction Type
17	Reference
1	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
61	Substrates and Products (Substrate)
2	Subunits
23	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 1.13.11.45 - linoleate 11-lipoxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Gaeumannomyces graminis

-

285253

phenyl-Sepharose column chromatography

Gaeumannomyces graminis

Q8X151

674584

recombinant His-tagged enzyme from Escherichia coli strain BL21 Star by nickel affinity chromatography, gel filtration, and ultrafiltration

Rippkaea orientalis PCC 8801

B7JX99

743826

recombinant mini 13R-MnLOX from Pichia pastoris by hydrophobic interaction chromatography and gel filtration

Gaeumannomyces graminis

-

725486

recombinant secreted enzyme 43fold from Pichia pastoris by hydrophobic interaction chromatography and gel filtration

Pyricularia oryzae

G4NAP4

741777

recombinant secreted wild-type and mutant enzymes from Pichia pastoris by hydrophobic interaction chromatography, ultrafiltration, and gel filtration

Fusarium oxysporum

-

743055

recombinant wild-type and mutant enzymes from Pichia pastoris by hydrophobic interaction chromatography, dialfiltration, and gel filtration

Gaeumannomyces graminis

-

725658

recombinant wild-type and mutant enzymes from Pichia pastoris cell medium by hydrophobic interaction and ion exchange chromatography

Gaeumannomyces graminis

-

688224