1.13.11.41: 2,4'-dihydroxyacetophenone dioxygenase
This is an abbreviated version!
For detailed information about 2,4'-dihydroxyacetophenone dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.41
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1.13.11.41
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cupin
-
alcaligenes
-
homotetramer
-
formic
-
4-hydroxybenzoic
-
ketone
-
chymotrypsinolysis
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tridentate
-
biomimetic
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nonhaem
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non-heme
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2-hydroxyacetophenone
-
equiv
- 1.13.11.41
-
cupin
- alcaligenes
-
homotetramer
-
formic
-
4-hydroxybenzoic
- ketone
-
chymotrypsinolysis
-
tridentate
-
biomimetic
-
nonhaem
-
non-heme
- 2-hydroxyacetophenone
-
equiv
Reaction
Synonyms
(4-hydroxybenzoyl)methanol oxygenase, DAD, DHAP dioxygenase
ECTree
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Purification
Purification on EC 1.13.11.41 - 2,4'-dihydroxyacetophenone dioxygenase
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recombinant enzyme 39fold from Escherichia coli strain JM109 by heat treatment, ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, native enzyme 17fold from strain AZ11 by gel filtration, anion exchange and hydrophobic interaction chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the His-tag by thrombin cleavage