1.13.11.4: gentisate 1,2-dioxygenase
This is an abbreviated version!
For detailed information about gentisate 1,2-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.4
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1.13.11.4
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maleylpyruvate
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protocatechuate
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3-hydroxybenzoate
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pseudaminobacter
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salicylatoxidans
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1-hydroxy-2-naphthoate
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3,4-dioxygenase
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monohydroxylated
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fumarylpyruvate
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isofunctional
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5-hydroxylase
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ring-fission
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ring-cleaving
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extradiol
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bicupins
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nocardioides
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acidovorans
- 1.13.11.4
- maleylpyruvate
- protocatechuate
- 3-hydroxybenzoate
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pseudaminobacter
- salicylatoxidans
- 1-hydroxy-2-naphthoate
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3,4-dioxygenase
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monohydroxylated
- fumarylpyruvate
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isofunctional
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5-hydroxylase
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ring-fission
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ring-cleaving
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extradiol
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bicupins
- nocardioides
- acidovorans
Reaction
Synonyms
2,5-dihydroxybenzoate dioxygenase, DsmD, EC 1.13.1.4, EC 1.99.2.4, eGDO, GDO, GDO-II, gdoA, GDOsp, GenH, gentisate dioxygenase, gentisate oxygenase, gentisate-1,2-dioxygenase, gentisic acid oxidase, gtdA-2, hbzE, Nagl1, Nagl2, Nagl3, NarI, oxygenase, gentisate 1,2-di-, P25X gentisate 1,2-dioxygenase, P35X gentisate 1,2-dioxygenase, salicylate 1,2-dioxygenase, SDO
ECTree
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Storage Stability
Storage Stability on EC 1.13.11.4 - gentisate 1,2-dioxygenase
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-20°C, addition of glycerol and beta-mercaptoethanol, 96 h, 25% of activity remains
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 3.5% residual activity
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 42.9% residual activity
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 42.5% residual activity
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 86.5% residual activity
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 23.1% residual activity
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-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 70.9% residual activity
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-80°C, 2 weeks, purified enzyme loses 50% of its initial activity. Addition of 2.0 mM GSH (or cysteine), 0.1 mM Fe(NH4)2(SO4)2*6H2O, and 10% glycerol stabilizes the activity
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-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year
4°C, MOPS buffer with no additives, 96 h, complete loss of activity
cell free extracts adsorbed on polyester filters, stable
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-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year
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-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year
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4°C, MOPS buffer with no additives, 96 h, complete loss of activity
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