Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.13.11.4: gentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about gentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.4

Reaction

2,5-Dihydroxybenzoate
+
O2
=
maleylpyruvate

Synonyms

2,5-dihydroxybenzoate dioxygenase, DsmD, EC 1.13.1.4, EC 1.99.2.4, eGDO, GDO, GDO-II, gdoA, GDOsp, GenH, gentisate dioxygenase, gentisate oxygenase, gentisate-1,2-dioxygenase, gentisic acid oxidase, gtdA-2, hbzE, Nagl1, Nagl2, Nagl3, NarI, oxygenase, gentisate 1,2-di-, P25X gentisate 1,2-dioxygenase, P35X gentisate 1,2-dioxygenase, salicylate 1,2-dioxygenase, SDO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.4 gentisate 1,2-dioxygenase

Specific Activity

Specific Activity on EC 1.13.11.4 - gentisate 1,2-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
23ºC, with gentistate as substrate, G56 mutant
0.08
-
23ºC, with lactate as substrate, wild-type enzyme
0.09
-
23ºC, with 2,5-xylenol as substrate, G56 mutant
0.11
-
23ºC, with 3-hydroxybenzoate as substrate, G56 mutant
0.45
-
crude extract, at 23°C
0.52
-
23ºC, with gentistate as substrate, wild-type enzyme
0.6
-
substrate: 2,5-dihydroxybenzoate, pH 7.4, 30°C
0.72
-
crude extract, at 23°C
0.75
-
23ºC, with 2,5-xylenol as substrate, wild-type enzyme
0.81
-
23ºC, with 3-hydroxybenzoate as substrate, wild-type enzyme
1.2
pH 7.0, 22°C, extract from cells grown with benzoic acid
1.4
pH 7.0, 22°C, extract from cells grown with 3-phenylpropionic acid
1.5
pH 7.0, 22°C, extract from cells grown with cinnamic acid
145
recombinant enzyme, pH 7.4, 30°C
15.4
-
substrate: 3-chlorogentisate, pH 7.4, 30°C
23.2
-
after 51.56fold purification, at 23°C
43.14
-
after 59.92fold purification, at 23°C
additional information