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1.13.11.34: arachidonate 5-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 5-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.34

Reaction

(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
=
leukotriene A4
 +
H2O

Synonyms

5-lipoxygenase, 5-LO, 5-LO1, 5-LOX, 5DELTA-lipoxygenase, 5LO, 5LOX-1, ALOX5, arachidonate 5-LO, arachidonate:oxygen oxidoreductase, arachidonic 5-lipoxygenase, arachidonic acid 5-lipoxygenase, C-5-lipoxygenase, DELTA5-lipoxygenase, H5-LO, leukotriene A4 synthase, leukotriene-A4 synthase, lipoxygenase 15, lipoxygenase 5, lipoxygenase-1, LO-1, LOX-15, LOX-5, LTA synthase, LTA4 synthase, oxygenase, arachidonate, 5-lip-, PMNL 5-lipoxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.34 arachidonate 5-lipoxygenase

Purification

Purification on EC 1.13.11.34 - arachidonate 5-lipoxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immunoaffinity purification
-
major isoenzyme L1
-
MonoQ column chromatography
-
native enzyme to homogeneity from ungerminated seed
-
partial
partially purified by sonication, centrifugation and gel filtration
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21 by ATP affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and anion exchange chromatography
recombinant enzyme partially from Escherichia coli strain MV1190 by ammonium sulfate fractionation and ATP affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta2 by cobalt affinity chromatography and ultrafiltration
recombinant His-tagged wild-type enzyme and enzyme mutant from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, isolated enzyme PLAT domain
recombinant soluble 5-LODELTA4 mutant and wild-type enzyme 5-LO from Escherichia coli strain BL21(DE3) by ATP affinity chromatography and anion exchange chromatography
recombinant soluble MBP-regulatory C2-like domain fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, fusion protein cleavage by TEV protease, and further purification of the C2-like domain by hydrophobic interaction chromatography
-
using Ni-NTA chromatography
-