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1.13.11.34: arachidonate 5-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 5-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.34

Reaction

(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
=
leukotriene A4
 +
H2O

Synonyms

5-lipoxygenase, 5-LO, 5-LO1, 5-LOX, 5DELTA-lipoxygenase, 5LO, 5LOX-1, ALOX5, arachidonate 5-LO, arachidonate:oxygen oxidoreductase, arachidonic 5-lipoxygenase, arachidonic acid 5-lipoxygenase, C-5-lipoxygenase, DELTA5-lipoxygenase, H5-LO, leukotriene A4 synthase, leukotriene-A4 synthase, lipoxygenase 15, lipoxygenase 5, lipoxygenase-1, LO-1, LOX-15, LOX-5, LTA synthase, LTA4 synthase, oxygenase, arachidonate, 5-lip-, PMNL 5-lipoxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.34 arachidonate 5-lipoxygenase

Cloned

Cloned on EC 1.13.11.34 - arachidonate 5-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
5-lipoxygenase cDNA cloned into EGFP fusion protein vector (pEGFP-h5-LO) and expressed in HEK293/T or COS-7 cells, or into a pUC13 vector, full length 5-lipoxygenase cDNA subcloned into pGEX and pcDNA3, expressed in Escherichia coli Y1090
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detection of alternatively spliced 5-LO transcripts in B and T cell lines and in primary cells encoding naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, that lack the exons 13, 4 or a part of exon 12, respectively, RT-PCR expression analysis of 5-LO-WT and isoform expression. Coexpression of the truncated natural isoforms with 5-LO-wild-type in transiently and stably transfected HEK-293T cells
DNA and amino acid sequence analysis
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Escherichia coli MV1190 transformed with pT3-5LO plasmid
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expressed in Escherichia coli
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expressed in Escherichia coli as a His-tagged fusion protein
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expressed in Escherichia coli BL21 and MV1190 cells
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expressed in Sf-21 insect cells
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expression in Escherichia coli
expression in Escherichia coli strain MV1190
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expression in HEK-293 cell
expression in HEK-293 cells
expression in human osteosarcoma cell line 143.98.2
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expression of regulatory C2-like domain of the enzyme, residues 1-128, containing a tobacco etch virus protease-cleavage site, in Escherichia coli strain BL21(DE3) as soluble maltose-binding-protein fusion protein
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from adipose tissue, expression of the enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, subcloning and propagation of the virus in Escherichia coli strain DH10
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gene 5-LOX, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
gene Alox5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative realtime PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ALOX5, located on chromosome 10q11.21, it spans a region of 71.9 kbp and consists of 13 introns named A-M and 14 exons, semiquantitative PCR enzyme expression analysis. Recombinant expression of enzyme mutant 5-LODELTA4 in HEK-293T cells from expression plasmid, recombinant expression of soluble 5-LODELTA4 mutant and of wild-type enzyme 5-LO in Escherichia coli strain BL21(DE3)
gene alox5, recombinant expression in Escherichia coli strain BL21
gene ALOX5, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta2
gene ALOX5, recombinant expression of wild-type and mutant enzymes in HeLa cells, HEK-293 cells, and in Escherichia coli strain BL21 (DE3)
genotyping of 1916 sputum-positive patients with pulmonary tuberculosis from Ghana and in 2269 exposed, apparently healthy control individuals, polymorphisms of a variable number of tandem repeats of the ALOX5 promoter and of the exonic non-synonymous variant g.760G>A are analyzed by fragment length determination and fluorescence resonance energy transfer, respectively, and DNA sequencing. ALOX5 gene expression depends on the copy number of the Sp1-binding sequences in the promoter, whereby the 5 allele, compared with the 3, 4 and 6 alleles, is associated with the highest degree of expression
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HEK 293 cells stably transfected with a pcDNA3.1 vector expressing 5-lipoxygenase
-
high expression baculovirus system
-
recombinant expression of enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type enzyme, enzyme mutant, and isolated enzyme PLAT domain in Escherichia coli strain BL21(DE3)
the gene contains 14 exons, DNA methylation is responsible for suppression of 5-LO expression, the 5-LO core promoter is completely methylated in the cell lines U-937 and HL-60TB, which do not express 5-LO protein, regulation mechanisms, overview, expression in HeLa cells, which lack endogenous enzyme, for promoter analysis, the basal promoter undergoes DNA looping to distant gene regions
wild-type and mutant enzyme F359W/A424I/N425M/A603I, expression in Escherichia coli
-