1.13.11.3: protocatechuate 3,4-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.3

1.13.11.3

catecholate

1,2-dioxygenase

intradiol

4-hydroxybenzoate

3,4-dihydroxybenzoate

beta-ketoadipate

ring-cleavage

gentisate

beta-carboxy-cis,cis-muconate

enol-lactone

hydroxyquinol

orville

lipscomb

fuscum

cis-muconate

4-methylcatechols

3,5-di-tert-butylcatechol

analysis

environmental protection

degradation

Reaction

Synonyms

3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, PCD, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.11 With incorporation of two atoms of oxygen

1.13.11.3 protocatechuate 3,4-dioxygenase

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Results	in table
9	Activating Compound
6616	AA Sequence
5	Application
1	CAS Registry Number
15	Cloned(Commentary)
12	Crystallization (Commentary)
9	Expression
11	General Information
8	General Stability
3	IC50 Value
135	Inhibitors
3	kcat/KM [mM/s]
42	Ki Value [mM]
58	KM Value [mM]
1	Localization
21	Metals/Ions
42	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
123	Organism
12	Pathway
386	PDB ID
24	pH Optimum
9	pH Range
4	pH Stability
1	Posttranslational Modification
21	Protein Variants
31	Purification (Commentary)
5	Reaction
3	Reaction Type
89	Reference
5	Source Tissue
30	Specific Activity [micromol/min/mg]
10	Storage Stability
174	Substrates and Products (Substrate)
23	Subunits
50	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
16	Temperature Stability [°C]
41	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase

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20 - 50

Pseudomonas oleovorans

purified enzyme, 50 min, pH 7.5, relatively stable

725655

Nocardia erythropolis

1 h, stable below

439502

Neurospora crassa

stable for long periods

60 - 70

A0A193DXA9; A0A193DXP2

after incubating for 330 min at 60°C, the free enzyme maintains approximately 17% of its activity, and its half-life is 180 min. The enzyme retains 75% activity after 4 h at 70°C and shows no activity after 4 h at 90°C

741660

Pseudomonas aeruginosa

soluble and immobilized enzyme, 15 min, complete loss of activity

439485

Pseudomonas oleovorans

purified enzyme, 20 min, pH 7.5, loss of 95% activity

725655

Pseudomonas putida

the free enzyme loses 82% of relative activity after 180 min of incubations at 90°C

743818