1.13.11.3: protocatechuate 3,4-dioxygenase
This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.3
-
1.13.11.3
-
catecholate
-
1,2-dioxygenase
-
intradiol
-
4-hydroxybenzoate
-
3,4-dihydroxybenzoate
-
beta-ketoadipate
-
ring-cleavage
-
gentisate
-
beta-carboxy-cis,cis-muconate
-
enol-lactone
-
hydroxyquinol
-
orville
-
lipscomb
-
fuscum
-
cis-muconate
-
4-methylcatechols
-
3,5-di-tert-butylcatechol
-
analysis
-
environmental protection
-
degradation
- 1.13.11.3
-
catecholate
-
1,2-dioxygenase
-
intradiol
- 4-hydroxybenzoate
- 3,4-dihydroxybenzoate
-
beta-ketoadipate
-
ring-cleavage
- gentisate
- beta-carboxy-cis,cis-muconate
-
enol-lactone
- hydroxyquinol
-
orville
-
lipscomb
- fuscum
-
cis-muconate
- 4-methylcatechols
- 3,5-di-tert-butylcatechol
- analysis
- environmental protection
- degradation
Reaction
Synonyms
3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, PCD, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase
ECTree
1.13.11.3 protocatechuate 3,4-dioxygenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxyphenylalanine + O2
?
Nocardia erythropolis
-
at 10.7% the rate of protocatechuic acid oxidation
-
-
?
3-(3,4-dihydroxyphenyl)propionate + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
5-fluoro-protocatechuic acid + O2
5-fluoro-3-carboxy-cis,cis-muconate
-
at 2.1% the rate of protocatechuic acid oxidation
-
?
6-chloro-protocatechuate + O2
6-chloro-3-carboxy-cis,cis-muconate
-
at 4.3% the rate of protocatechuic acid oxidation
-
?
2,3-dihydroxybenzoate + O2
?
23.6% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,3-dihydroxybenzoate + O2
?
23.6% of the activity with protocatechuate for the free enzyme, 149.3% for the calcium alginate-immobilized enzyme, and 119.8% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,4-dihydroxybenzoate + O2
?
43.5% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + O2
?
43.5% of the activity with protocatechuate for the free enzyme, 96.5% for the calcium alginate-immobilized enzyme, and 65.6% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,5-dihydroxybenzoate + O2
?
33.46% activity compared to 3,4-dihydroxybenzoate
-
-
?
2,5-dihydroxybenzoate + O2
?
33.5% of the activity with protocatechuate for the free enzyme, 158.6% for the calcium alginate-immobilized enzyme, and 72.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,6-dihydroxybenzoate + O2
?
30.5% of the activity with protocatechuate for the free enzyme, 90.4% for the calcium alginate-immobilized enzyme, and 36.2% for the glyoxyl agarose-immobilized enzyme
-
-
?
2,6-dihydroxybenzoate + O2
?
30.51% activity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
absolute requirement for vicinal hydroxyl groups in the 3- and 4-position
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 2-chloro-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 5-bromo-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 5-chloro-protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
methylene blue cannot replace O2 as electron acceptor
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
Nocardia erythropolis
-
enzyme is active on a wide range of o-dihydroxyphenyl compounds
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with: 3,4-dihydroxyphenylacetic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 3,4-dihydroxymandelic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 4'-methylcatechol
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with: 3,4-dihydroxyphenylacetic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 3,4-dihydroxymandelic acid
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
very low activity with 4'-methylcatechol
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
ir
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
i.e. protocatechuate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
specific for protocatechuate
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
best substrate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
100% activity
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
100% activity
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
best substrate
-
-
?
3,4-dihydroxybenzoate + O2
3-carboxy-cis,cis-muconate
-
no other substrate found
-
?
3,4-dihydroxybenzoate + O2
?
-
spectroscopic and electronic structure study of the enzyme-substrate complex
-
-
?
3,4-dihydroxybenzoate + O2
?
-
spectroscopic and electronic structure study of the enzyme-substrate complex
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.54% ctivity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.54% ctivity compared to 3,4-dihydroxybenzoate
-
-
?
3,4-dihydroxyhydrocinnamic acid + O2
?
29.5% of the activity with protocatechuate for the free enzyme, 95.0% for the calcium alginate-immobilized enzyme, and 41.1% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,4-dihydroxymandelic acid + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
3,4-dihydroxymandelic acid + O2
?
-
protocatechuate 3,4-dioxygenase II
-
-
?
3,4-dihydroxymandelic acid + O2
?
Nocardia erythropolis
-
at 5.4% the rate of protocatechuic acid oxidation
-
-
?
3,4-dihydroxyphenylacetate + O2
?
-
W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 13% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate
-
-
?
3,5-dihydroxybenzoate + O2
?
44.3% of the activity with protocatechuate for the free enzyme, 113.3% for the calcium alginate-immobilized enzyme, and 42.9% for the glyoxyl agarose-immobilized enzyme
-
-
?
3,5-dihydroxybenzoate + O2
?
44.33% activity compared to 3,4-dihydroxybenzoate
-
-
?
3-methylcatechol + O2
2-methylmuconate
-
at 5% the rate of protocatechuic acid oxidation
-
?
3-methylcatechol + O2
2-methylmuconate
Nocardia erythropolis
-
at 14.4% the rate of protocatechuic acid oxidation
-
?
3-methylcatechol + O2
2-methylmuconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
-
at very low rates
-
?
4-methylcatechol + O2
3-methyl-cis,cis-muconate
Nocardia erythropolis
-
at 3.1% the rate of protocatechuic acid oxidation
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
4-sulfocatechol + O2
3-sulfomuconate
-
catalyzed by protocatechuate 3,4-dioxygenase type II only
-
?
caffeic acid + O2
?
34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme
-
-
?
caffeic acid + O2
?
34.21% activity compared to 3,4-dihydroxybenzoate
-
-
?
caffeic acid + O2
?
34.21% activity compared to 3,4-dihydroxybenzoate
-
-
?
caffeic acid + O2
?
34.2% of the activity with protocatechuate for the free enzyme, 150% for the calcium alginate-immobilized enzyme, and 97% for the glyoxyl agarose-immobilized enzyme
-
-
?
catechol + O2
muconate
-
at 3% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
Nocardia erythropolis
-
at 33.8% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
catechol + O2
muconate
-
at 0.4% the rate of protocatechuic acid oxidation
-
?
pyrogallol + O2
?
-
at 10% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
Nocardia erythropolis
-
at 36% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
-
at 0.4% the rate of protocatechuic acid oxidation
-
-
?
pyrogallol + O2
?
-
at 0.4% the rate of protocatechuic acid oxidation
-
-
?
trans-3,4-dihydroxycinnamate + O2
?
-
W153V protocatechuate 3,4-dioxygenase I mutant enzyme: 35% activity compared to protocatechuate, wild-type protocatechuate 3,4-dioxygenase I: 2% activity compared to protocatechuate
-
-
?
additional information
?
-
benzoate induces expression of protocatechuate 3,4-dioxygenase
-
-
?
additional information
?
-
benzoate induces expression of protocatechuate 3,4-dioxygenase
-
-
?
additional information
?
-
-
enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway
-
-
?
additional information
?
-
-
the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources
-
-
?
additional information
?
-
Pseudomonas oleovorans KF707
-
the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources
-
-
?
additional information
?
-
-
strain KB2 degrades 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation
-
-
?
additional information
?
-
protocatechuate 3,4-dioxygenase from KB2 strain shows activity against various dihydroxybenzoic acids, but highest activity with primary substrate protocatechuate. Activity of the enzyme immobilized on calcium alginate increases particularly towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate, overview
-
-
?
additional information
?
-
-
protocatechuate 3,4-dioxygenase from KB2 strain shows activity against various dihydroxybenzoic acids, but highest activity with primary substrate protocatechuate. Activity of the enzyme immobilized on calcium alginate increases particularly towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate, overview
-
-
?
additional information
?
-
-
strain KB2 degrades 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation
-
-
?
additional information
?
-
protocatechuate 3,4-dioxygenase from KB2 strain shows activity against various dihydroxybenzoic acids, but highest activity with primary substrate protocatechuate. Activity of the enzyme immobilized on calcium alginate increases particularly towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate, overview
-
-
?
