1.13.11.3: protocatechuate 3,4-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 3,4-dioxygenase, go to the full flat file.

Reaction

Synonyms

3,4-PCase, 3,4-PCD, 3,4-PCDase, 3,4-POD, EC 1.13.1.3, EC 1.99.2.3, More, oxygenase, protocatechuate 3,4-di-, P3,4DO, P3,4O enzyme, P34O, PCA 3,4-dioxygenase, PcaG, PcaH, PcaHG, PCD, protocatchetuate 3,4-dioxygenase, protocatechuate 3,4-dioxygenase, protocatechuate oxygenase, protocatechuic 3,4-dioxygenase, protocatechuic 3,4-oxygenase, protocatechuic acid oxidase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.3 protocatechuate 3,4-dioxygenase

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Results	in table
9	Activating Compound
6616	AA Sequence
5	Application
1	CAS Registry Number
15	Cloned(Commentary)
12	Crystallization (Commentary)
9	Expression
11	General Information
8	General Stability
3	IC50 Value
135	Inhibitors
3	kcat/KM [mM/s]
42	Ki Value [mM]
58	KM Value [mM]
1	Localization
21	Metals/Ions
42	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
123	Organism
12	Pathway
386	PDB ID
24	pH Optimum
9	pH Range
4	pH Stability
1	Posttranslational Modification
21	Protein Variants
31	Purification (Commentary)
5	Reaction
3	Reaction Type
89	Reference
5	Source Tissue
30	Specific Activity [micromol/min/mg]
10	Storage Stability
174	Substrates and Products (Substrate)
23	Subunits
50	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
16	Temperature Stability [°C]
41	Turnover Number [1/s]

KI Value

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KI Value on EC 1.13.11.3 - protocatechuate 3,4-dioxygenase

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.29

2,5-Dihydroxybenzoate

Acinetobacter calcoaceticus

-

-

439500

0.01

2-Fluoro-4-hydroxybenzoate

Pseudomonas putida

-

-

439486

0.0068

2-Hydroxyisonicotinic acid N-oxide

Pseudomonas sp.

-

apparent Ki, irreversible binding

439494

3.9

2-Hydroxypyridine N-oxide

Pseudomonas sp.

-

apparent Ki, inhibition is not freely reversible

439494

0.13

3,4-Dihydroxyacetophenone

Pseudomonas putida

-

-

439486

0.013

3,4-Dihydroxyphenylacetate

Pseudomonas putida

-

-

439486

0.041

3,4-dihydroxyphenylacetic acid

Acinetobacter calcoaceticus

-

-

439500

0.018

3,4-Dihydroxyphenylpropionate

Pseudomonas putida

-

-

439486

0.044

3,5-Dichloro-4-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.018

3-Bromo-4-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.0032

3-Chloro-4-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.0003 - 0.01

3-Fluoro-4-hydroxybenzoate

3 entries

0.004

3-hydroxybenzoate

Pseudomonas putida

-

-

439486

0.3 - 0.8

4-Fluoro-3-hydroxybenzoate

2 entries

0.087 - 0.24

4-hydroxybenzoate

3 entries

0.44

4-methylcatechol

Acinetobacter calcoaceticus

-

-

439500

0.0006 - 0.0048

4-nitrocatechol

2 entries

0.032 - 0.053

4-sulfocatechol

2 entries

0.17

alpha-chloro-3,4-dihydroxyacetophenone

Acinetobacter calcoaceticus

-

-

439500

0.38 - 0.7

catechol

2 entries

30

HCN

Brevibacterium fuscum

-

-

439497

1.3

isonicotinic acid N-oxide

Pseudomonas sp.

-

-

439494

0.02

Isovanillate

Pseudomonas putida

-

-

439486

0.001 - 0.073

Protocatechualdehyde

4 entries

0.066 - 0.102

protocatechuate

2 entries

0.4

protocatechuic acid methylester

Pseudomonas putida

-

-

439486

0.003

vanillate

Pseudomonas putida

-

-

439486

additional information

additional information

3 entries

-

0.0003

3-Fluoro-4-hydroxybenzoate

Pseudomonas putida

-

at pH 7.0

439487

0.0005

3-Fluoro-4-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.01

3-Fluoro-4-hydroxybenzoate

Pseudomonas putida

-

-

439486

0.3

4-Fluoro-3-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.8

4-Fluoro-3-hydroxybenzoate

Pseudomonas putida

-

-

439486

0.087

4-hydroxybenzoate

Pseudomonas putida

-

at pH 8.4

439487

0.1

4-hydroxybenzoate

Pseudomonas aeruginosa

-

-

439495

0.24

4-hydroxybenzoate

Pseudomonas putida

-

-

439486

0.0006

4-nitrocatechol

Azotobacter vinelandii

-

-

439501

0.0048

4-nitrocatechol

Acinetobacter calcoaceticus

-

-

439500

0.032

4-sulfocatechol

Hydrogenophaga palleronii

-

protocatechuate 3,4-dioxygenase type II

439505

0.053

4-sulfocatechol

Agrobacterium tumefaciens

-

protocatechuate 3,4-dioxygenase type II

439505

0.38

catechol

Acinetobacter calcoaceticus

-

-

439500

0.7

catechol

Pseudomonas putida

-

-

439486

0.001

Protocatechualdehyde

Azotobacter vinelandii

-

-

439501

0.014

Protocatechualdehyde

Pseudomonas aeruginosa

-

-

439483

0.014

Protocatechualdehyde

Pseudomonas putida

-

-

439486

0.073

Protocatechualdehyde

Acinetobacter calcoaceticus

-

-

439500

0.066

protocatechuate

Hydrogenophaga palleronii

-

protocatechuate 3,4-dioxygenase type II

439505

0.102

protocatechuate

Agrobacterium tumefaciens

-

protocatechuate 3,4-dioxygenase type II

439505

additional information

additional information

Pseudomonas putida

-

rates (s-1) and dissociation constants (microM) for 4-hysroxybenzoate: wild type: k+2 = 42, k-2 = 14, k+2 + k-2 = 56, K1 = 700, Kd = 170, Y408H: k+2 + k-2 = 2.3, Y408C: k+2 + k-2 = 0.7, Y408F: k+2 + k-2 = 0.9

671966

-

additional information

additional information

Pseudomonas putida

-

substrate dissociation constant for 3-hydroxybenzoate: wild type = 3500 microM, Y408H = 180 microM, Y408E = 210 microM, Y408C = 2500 microM, Y408F = 1800 microM

671966

-

additional information

additional information

Pseudomonas putida

-

substrate dissociation constant for 4-hydroxybenzoate: wild type = 300 microM, Y408H = 6.3 microM, Y408C = 51 microM, Y408F = 12 microM

671966

-