1.13.11.20: cysteine dioxygenase

This is an abbreviated version!
For detailed information about cysteine dioxygenase, go to the full flat file.

Reaction

Synonyms

3-mercaptopropionate dioxygenase, 3MDO, ADO, Arg-type CDO, BsCDO, CDO, CDO1, CDO2, CdoA, CdoB, cysteine dioxygenase, cysteine dioxygenase type 1, cysteine oxidase, Fe(II) cysteine dioxygenase, H16_A1614, H16_B1863, NP_251292, oxygenase, cysteine di-, PA2602, PCO1, PCO4

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.20 cysteine dioxygenase

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Results	in table
42	Activating Compound
5409	AA Sequence
3	Application
1	CAS Registry Number
37	Cloned(Commentary)
11	Cofactor
15	Crystallization (Commentary)
131	Disease/ Diagnostics
5	Expression
64	General Information
1	General Stability
95	Inhibitors
16	kcat/KM [mM/s]
3	Ki Value [mM]
46	KM Value [mM]
5	Localization
63	Metals/Ions
26	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
114	Organism
3	Oxidation Stability
7	Pathway
85	PDB ID
24	pH Optimum
1	pH Range
4	pI Value
8	Posttranslational Modification
27	Protein Variants
32	Purification (Commentary)
10	Reaction
4	Reaction Type
104	Reference
65	Source Tissue
23	Specific Activity [micromol/min/mg]
4	Storage Stability
128	Substrates and Products (Substrate)
23	Subunits
85	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
31	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.003 - 0.033

3-Mercaptopropanoate

9 entries

0.82

3-Mercaptopropionate

Cupriavidus necator

Q0K029, Q0KB75

pH 6.5, 30°C, recombinant His-tagged enzyme

741704

0.29

cysteamine

Azotobacter vinelandii

-

pH 8.0, 25°C

758785

0.01 - 10.35

L-cysteine

16 entries

20.1

N-terminal Cys of RGS4

Homo sapiens

Q96SZ5

pH not specified in the publication, 37°C

765832

-

16.9

N-terminal Cys of RGS5

Homo sapiens

Q96SZ5

pH not specified in the publication, 37°C

765832

-

41.2 - 56.6

O2

2 entries

0.003

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

mutant G95C, PIPES buffer pH 6.5, 37°C

758787

0.01

3-Mercaptopropanoate

Cupriavidus necator

Q0K029, Q0KB75

pH 6.5, 30°C

741704

0.011

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, PIPES buffer pH 6.1, 37°C

758787

0.013

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, phosphate buffer pH 8.0, 37°C

758787

0.019

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, PIPES buffer pH 7.5, 37°C

758787

0.021

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

pH 7.5, 37°C

759471

0.021

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, phosphate buffer pH 7.5, 37°C

758787

0.022

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, PIPES buffer pH 6.5, 37°C

758787

0.033

3-Mercaptopropanoate

Pseudomonas aeruginosa

Q9I0N5

wild-type, PIPES buffer pH 7.0, 37°C

758787

0.01

L-cysteine

Cupriavidus necator

Q0K029, Q0KB75

pH 6.5, 30°C, recombinant His-tagged enzyme

741704

0.012

L-cysteine

Rattus norvegicus

-

assay standart conditions are: the enzyme is incubated at 37°C in the presence of 62.5 mM Mes buffer (pH 6.1), 0.3 mM ferrous sulfate, 0.0125 mM bathocuproine disulfonate and 1.2 mM cysteine

676962

0.12

L-cysteine

Rattus norvegicus

P21816

20% cross-linked wild-type enzyme, pH 9.1, temperature not specified in the publication

741904

0.25

L-cysteine

Rattus norvegicus

P21816

60% cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication

741904

0.25

L-cysteine

Cupriavidus necator

Q0K029, Q0KB75

pH 6.5, 30°C, recombinant His-tagged enzyme

741704

0.3

L-cysteine

Streptomyces coelicolor

O50490, Q9KZL0

SCO5772

674292

0.33

L-cysteine

Streptomyces coelicolor

O50490, Q9KZL0

SCO3035

674292

0.33

L-cysteine

Rattus norvegicus

P21816

not cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication

741904

0.39

L-cysteine

Bacillus subtilis

O32085

YubC

674292

0.62

L-cysteine

Rattus norvegicus

P21816

-

674292

1.2

L-cysteine

Azotobacter vinelandii

-

pH 8.0, 25°C

758785

1.7

L-cysteine

Homo sapiens

Q16878

determind under saturating oxygen conditions

674873

1.8

L-cysteine

Mus musculus

P60334

presence of 0.1 mol Fe2+, absence of EDTA

676850

2

L-cysteine

Bacillus cereus

Q81CX4

-

674292

3.6

L-cysteine

Mus musculus

P60334

presence of 0.1 mol Fe2+, absence of EDTA

676850

10.35

L-cysteine

Homo sapiens

Q16878

pH 6.1, 37°C, recombinnat wild-type enzyme

741585

41.2

O2

Homo sapiens

Q96SZ5

cosubstrate N-terminal Cys of RGS5, pH not specified in the publication, 37°C

765832

56.6

O2

Homo sapiens

Q96SZ5

cosubstrate N-terminal Cys of RGS4, pH not specified in the publication, 37°C

765832