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1.13.11.2: catechol 2,3-dioxygenase

This is an abbreviated version!
For detailed information about catechol 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.2

Reaction

catechol
+
O2
=
2-hydroxymuconate-6-semialdehyde

Synonyms

2,3-dihydroxybenzenesulfonate 2,3-dioxygenase, 3-sulfocatechol-2,3-dioxygenase, 3SC23O, A23O, AtdB, BupB, C2,3O, C23D, C23o, C23O-2G, C23O1, C23O2, C23Os, CatE, catechol 2,3 dioxygenase, catechol 2,3-di-2,3-pyrocatechase, catechol 2,3-dioxygenase, catechol 2,3-oxygenase, catechol oxygenase, catechol-2,3-dioxygenase, CbzE, CD-2,3, EC 1.13.1.2, ECDO, extradiol dioxygenase MhpB, Extradiol-cleaving catecholic dioxygenase, meta-cleavage dioxygenase, metapyrocatechase, More, oxygenase, PheB, pyrocatechol 2,3-dioxygenase, Saci_2295, SSO1223, SsoC2,3O, ssol_2912, tbuE, TdnC, TodE, XylE

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.2 catechol 2,3-dioxygenase

Specific Activity

Specific Activity on EC 1.13.11.2 - catechol 2,3-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00073
mutant enzyme A23T/F212S, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.0009
mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00108
mutant enzyme H24R/F168S/Q275R, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00125
wild type enzyme, with 3-methylcatechol as substrate, at pH 7.0 and 35°C
0.00138
mutant enzyme R296Q, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00155
mutant enzyme M65T, with 3-methylcatechol as substrate, at pH 7.0 and 35°C
0.0018
wild type enzyme, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00272
mutant enzyme A23T/F212S, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00298
mutant enzyme H24R/F168S/Q275R, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00547
mutant enzyme H24R/F168S/Q275R, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00572
mutant enzyme H24R/F168S/Q275R, with catechol as substrate, at pH 7.5, temperature not specified in the publication
0.00806
mutant enzyme A23T/F212S, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.00811
mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication
0.0082
Halalkalibacterium ligniniphilum
WP_017726464.1
substrate: catechol, pH 7.4, 32.5°C
0.00827
mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with catechol as substrate, at pH 7.5, temperature not specified in the publication
0.00878
mutant enzyme A23T/F212S, with catechol as substrate, at pH 7.5, temperature not specified in the publication
0.00887
-
pH 7.0, 35°C, mutant H24R/F168S/Q275R
0.00938
wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C
0.00963
wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication
0.01
-
inductor 3,4-dihydroxybenzoic acid, cell-free extract from Stenotrophomonas maltophilia
0.01285
-
pH 7.0, 35°C, mutant A23T/F212S
0.01487
mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.018
-
Intracellular enzyme activity of C23D in crude lysate
0.01966
wild type enzyme, with catechol as substrate, at pH 7.5, temperature not specified in the publication
0.01973
-
pH 7.0, 35°C, wild-type enzyme
0.02
-
inductor benzoic acid, cell-free extract from Stenotrophomonas maltophilia
0.02062
wild type enzyme, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.02065
wild type enzyme, with catechol as substrate, at pH 7.0 and 35°C
0.0214
wild type enzyme, with 4-methylcatechol as substrate, at pH 7.0 and 35°C
0.02223
mutant enzyme R296Q, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication
0.02245
mutant enzyme M65T, with catechol as substrate, at pH 7.0 and 35°C
0.02775
mutant enzyme R296Q, with catechol as substrate, at pH 7.5, temperature not specified in the publication
0.0312
mutant enzyme M65T, with 4-methylcatechol as substrate, at pH 7.0 and 35°C
0.03187
-
pH 5.0, 35°C, mutant R296Q
0.04
-
inductor vanillic acid, cell-free extract from Stenotrophomonas maltophilia
0.04843
mutant enzyme M65T, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C
0.054
-
Activity is measured by following the formation of 2-hydroxymuconic semialdehyde spectrophotometrically at 375 nm
0.05605
mutant enzyme R296Q, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication
0.37
-
inductor catechol, cell-free extract from Stenotrophomonas maltophilia
10
with 2,3-dihydroxybiphenyl, pH 7.5, 28°C
15
crude extract, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C
27.2
pH 8.0, 30°C
270
-
free enzyme
300
-
highest specific activity at arount 40°C for both wild-type and mutated C23O
35
-
metapyrocatechase 2
355.8
-
substrate 4-methylcatechol, pH 8.0, 35°C, free enzyme
4.92
-
inductor phenol, cell-free extract from Stenotrophomonas maltophilia
400
-
wild-type enzyme
46
-
immobilized enzyme
536
-
crystallized enzyme
593
with 4-methylcatechol, pH 7.5, 28°C
6.9
-
crude extracts of Pseudomonas putida
60.3
-
crude extracts of Escherichia coli
68
with 3-methylcatechol, pH 7.5, 28°C
802
after 52.7fold purification, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C
90
-
metapyrocatechase 1
936.3
-
substrate 4-methylcatechol, pH 8.0, 35°C, immobilized enzyme
additional information