Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.13.11.2: catechol 2,3-dioxygenase

This is an abbreviated version!
For detailed information about catechol 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.2

Reaction

catechol
+
O2
=
2-hydroxymuconate-6-semialdehyde

Synonyms

2,3-dihydroxybenzenesulfonate 2,3-dioxygenase, 3-sulfocatechol-2,3-dioxygenase, 3SC23O, A23O, AtdB, BupB, C2,3O, C23D, C23o, C23O-2G, C23O1, C23O2, C23Os, CatE, catechol 2,3 dioxygenase, catechol 2,3-di-2,3-pyrocatechase, catechol 2,3-dioxygenase, catechol 2,3-oxygenase, catechol oxygenase, catechol-2,3-dioxygenase, CbzE, CD-2,3, EC 1.13.1.2, ECDO, extradiol dioxygenase MhpB, Extradiol-cleaving catecholic dioxygenase, meta-cleavage dioxygenase, metapyrocatechase, More, oxygenase, PheB, pyrocatechol 2,3-dioxygenase, Saci_2295, SSO1223, SsoC2,3O, ssol_2912, tbuE, TdnC, TodE, XylE

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.2 catechol 2,3-dioxygenase

Purification

Purification on EC 1.13.11.2 - catechol 2,3-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
50 mM Tris-SO4 buffer, pH 8.0, at 4 °C. Sucrose density gradient, two-step anion exchange chromatography using MonoQ column attached at a Dionex HPLC system.
-
cells are disrupted and centrifugates, protein supernatant is separated by 2D gel electrophoresis
-
DEAE-Sepharose column chromatography, phenyl-Superose gel filtration, and Sephacryl S-200 gel filtration
hydrophobic interaction column pre-equilibrated with 30% (w/v) ammonium sulfate and 0.1 M potassium phosphate buffer pH 7.5, protein eluation with ammonium sulfate gradient [30-0% (w/v)]. DEAE column pre-equilibrated with 50 mM potassium phosphate buffer pH 7.5, protein eluation with NaCl gradient (80-400 mM)
-
metapyrocatechase 1 and 2
-
Ni-NTA column chromatography
-
Ni-NTA-agarose column
-
purified 35.3fold with a yield of 46.1%
-
recombinant enzyme
recombinant protein
-
ultracentrifugation and Prepease nickel resin chromatography
-
wild-type and mutant enzymes
-