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1.13.11.18: persulfide dioxygenase

This is an abbreviated version!
For detailed information about persulfide dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.18

Reaction

S-sulfanylglutathione
+
O2
+
H2O
=
glutathione
+
sulfite
+ 2 H+

Synonyms

AFE_0269, BAE27_01805, BpPRF, cPDO-PT, CstB, ETHE1, ETHE1-like sulfur dioxygenase, Ethe2, Fe(II)-containing persulfide dioxygenase, Gly3, PDO, PRF, SDO, SOR, sulfide dioxygenase, sulfur dioxygenase, sulfur oxygenase, sulfur oxygenase/reductase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.18 persulfide dioxygenase

Cloned

Cloned on EC 1.13.11.18 - persulfide dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli HB101
-
expression in Escherichia coli
gene cst, CstB is encoded by the cst operon and is under the transcriptional control of the persulfide sensor CstR and H2S, recombinant expression of full-length wild-type and mutant enzymes and of the N-terminal PDO-RHD domain in Escherichia coli strain Rosetta (DE3)
gene ethe1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in Escherichia coli
gene sdo, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, recombinant overexpression in Escherichia coli strain BL21(DE3)
quantitative real-time RT-PCR enzyme expression analysis
-
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21
-