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evolution
DNA and amino acid sequence determination and analysis of LOX1 and LOX2 isozymes, phylogenetic analysis, only LOX1:Md:1a exhibits a glycine residue (Gly567) responsible for dual positional specificity and (R)-LOX activity
evolution
genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate
malfunction
deletion of lox greatly diminishes density-dependent development of both sclerotia and conidia, resulting in an overall increase in the number of sclerotia and a decrease in the number of conidia at high cell densities. Lox mutants show decreased linoleic acid LOX activity
malfunction
reduced LOX2 expression decreases the release of green leaf volatiles but does not impair jasmonic acid and jasmonic acid-Ile accumulation
metabolism
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depending on the regiospecificity of the enzyme, the incorporation of molecular oxygen leads to formation of 9- or 13-fatty acid hydroperoxides, which are used by LOX itself as well as by members of at least six different enzyme families to form a series of biologically active molecules, collectively called oxylipins
metabolism
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depending on the regiospecificity of the enzyme, the incorporation of molecular oxygen leads to formation of 9- or 13-fatty acid hydroperoxides, which are used by LOX itself as well as by members of at least six different enzyme families to form a series of biologically active molecules, collectively called oxylipins
metabolism
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13-hydroxy-9,11,5-octadecatrienoic acid (13-HOTrE) is produced by the reduction of a 13-LOX product, 13-hydroperoxy-9,11,15-octadecatrienoic acid (by peroxidases), one of the key substrates of jasmonate biosynthesis
metabolism
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profiling of oxylipins from young maize roots reveals complex patterns of products mainly originating from the combined actions of 9- and 13-lipoxygenases and allene oxide synthase. A distinctive feature is the high content of the cyclopentenone 10-oxo-11-phytoenoic acid (10-oxo-PEA)
metabolism
the enzyme is involved in the LOX pathway, overview
physiological function
9/13-LOX is associated with the ripening and senescence processes
physiological function
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expression of 15-LO-1 significantly decreases cell proliferation and increases apoptosis. Reduces adhesion to fibronectin, anchorage-independent growth on soft agar, cellular motility and ability to heal a scratch wound, and migratory and invasive capacity across Matrigel. 15-LO-1 expression reduces the expression of metastasis associated protein-1, a part of the nucleosome remodeling and histone deacetylase silencing complex
physiological function
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involvement of Cd-induced LOX activity in the premature differentiation of the barley root tip during Cd stress
physiological function
LOX-2 is a 13S-lipoxygenases, it has no dual positional specificity
physiological function
LOX-3 is a 13S-lipoxygenases, it has no dual positional specificity
physiological function
LOX-4 is a 13S-lipoxygenases, it has no dual positional specificity
physiological function
LOX-6 is a 13S-lipoxygenases, it has no dual positional specificity
physiological function
LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack
physiological function
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major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds
physiological function
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plant lipoxygenases catalyse the oxygenation of polyunsaturated fatty acids, linoleic and alpha-linolenic acid and are involved in processes such as stress responses and development
physiological function
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plant lipoxygenases catalyse the oxygenation of polyunsaturated fatty acids, linoleic and alpha-linolenic acid and are involved in processes such as stress responses and development
physiological function
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the enzyme is involved in endogenous JA synthesis and tolerance to biotic and abiotic stress
physiological function
lipoxygenase (LOX) is an important contributor to the formation of aroma-active C6 aldehydes in apple (Malus domestica) fruit upon tissue disruption, role in autonomously produced aroma volatiles from intact tissue, overview. The genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio- and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits, suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples. While isozymes LOX1:Md:1c, LOX2:Md:2a, and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption isozyme, LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. Both 9- and 13-hydroperoxides can be catabolized to aroma-active volatile aldehydes by hydroperoxide lyase. Only 13-LOX activity contributes to the apple aroma due to the formation of precursors of C6 volatile compounds. The dioxygenation of PUFAs by 9- and 13-LOX activity forms precursors for important phytooxylipins with functions in plant defense, wound signaling, senescence and fruit ripening
physiological function
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secreted lipoxygenase from Pseudomonas aeruginosa exhibits biomembrane oxygenase activity and induces hemolysis in human red blood cells. Secreted lipoxygenase oxygenates free arachidonic acid predominantly to 15S-hydro(peroxy)-5Z,8Z,11Z,13E-eicosatetraenoic acid. The enzyme is capable of binding phospholipids at its active site and physically interacts with model membranes
physiological function
the isozyme CmLOX10 is involved in biotic and abiotic stress
physiological function
the isozyme CmLOX13 is involved in biotic and abiotic stress
physiological function
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the lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (HPOD), which is important in food and flavour industries for production of bread and flavouring compounds
physiological function
15 lipoxygenase 1 is abundant in asthmatic human airway epithelial cells and binds phosphatidylethanolamine-binding protein 1 (PEBP1), leading to generation of hydroperoxy-phospholipids, which drive ferroptotic cell death. 15LO1, PEBP1, and glutathione peroxidase 4 GPX4 activity drives abnormal asthmatic redox biology, to enhance type 2 inflammatory responses. In vitro, type 2 inflammatory cytokine IL-13 induces 15LO1 generation of hydroperoxy-phospholipids, which lowers intracellular GSH and increased extracellular GSSG levels. Lowering GSH further by inhibiting cystine transporter SLC7A11 enhances type 2 inflammatory protein expression and ferroptosis. Ex vivo, redox imbalances correspond to 15LO1 and SLC7A11 expression, type 2 inflammatory biomarkers, and worsen clinical outcomes
physiological function
eosinophils are the major cell type expressing 12/15-LOX during the corneal wound healing process. Eosinophils are recruited into the conjunctiva after corneal epithelium wounding, and eosinophil-deficient and/or eosinophil-specific 12/15-LOX knockout mice show delayed corneal wound healing compared with wild-type mice. A series of 12/15-LOX-derived mediators are significantly decreased in eosinophil-deficient mice and topical application of 17-hydroxydocosahexaenoic acid restores the phenotype
physiological function
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the 13-lipoxygenase MSD2 does not function in herbivore-induced jasmonic acid production. The enzyme is important for producing jasmonic acid to regulate panicle development and spikelet fertility
physiological function
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the enzyme plays a role in berry lipid peroxidation and possibly oxylipins synthesis
physiological function
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the lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (HPOD), which is important in food and flavour industries for production of bread and flavouring compounds
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additional information
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non-heme iron lipoxygenase oxidizes C18-polyunsaturated fatty acids to 13S-hydroperoxy derivatives by an antarafacial reaction mechanism where the bis-allylic hydrogen abstraction is the rate-limiting step
additional information
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enzyme homology modeling using the crystal structure of linoleate 13-lipoxygenase from Pseudomonas aeruginosa, PDB entry 4G32, as a template
additional information
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non-heme iron lipoxygenase oxidizes C18-polyunsaturated fatty acids to 13S-hydroperoxy derivatives by an antarafacial reaction mechanism where the bis-allylic hydrogen abstraction is the rate-limiting step
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additional information
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enzyme homology modeling using the crystal structure of linoleate 13-lipoxygenase from Pseudomonas aeruginosa, PDB entry 4G32, as a template
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