1.12.98.1: coenzyme F420 hydrogenase

This is an abbreviated version!
For detailed information about coenzyme F420 hydrogenase, go to the full flat file.

Reaction

Synonyms

8-hydroxy-5-deazaflavin-reactive hydrogenase, 8-hydroxy-5-deazaflavin-reducing hydrogenase, coenzyme F420-dependent hydrogenase, coenzyme F420-reducing dehydrogenase, deazaflavin-reducing hydrogenase, EC 1.12.99.1, F420-reducing hydrogenase, F420-reducing [NiFe] hydrogenase, F420-reducing [NiFe]-hydrogenase, F420H2 dehydrogenase, Fpo, FRH, FrhABC, FrhABG, frhAGB-encoded hydrogenase, hydrogen:(acceptor) oxidoreductase, Mbar_A0449, Mbar_A0450, Mbar_A0452, TON_1559, TON_1560, TON_1561, VhuD

ECTree

     1 Oxidoreductases

         1.12 Acting on hydrogen as donor

             1.12.98 With other, known, physiological acceptors

                1.12.98.1 coenzyme F420 hydrogenase

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Results	in table
8	Activating Compound
1025	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
25	Cofactor
1	Crystallization (Commentary)
1	Expression
11	General Information
4	General Stability
5	Inhibitors
2	kcat/KM [mM/s]
1	Ki Value [mM]
20	KM Value [mM]
9	Localization
37	Metals/Ions
40	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
82	Organism
5	Oxidation Stability
6	Pathway
14	PDB ID
8	pH Optimum
5	pH Range
1	pH Stability
1	pI Value
2	Protein Variants
18	Purification (Commentary)
1	Reaction
3	Reaction Type
43	Reference
24	Specific Activity [micromol/min/mg]
6	Storage Stability
97	Substrates and Products (Substrate)
26	Subunits
39	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
10	Turnover Number [1/s]

Molecular Weight

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of the Enzyme Summary Page.

Molecular Weight on EC 1.12.98.1 - coenzyme F420 hydrogenase

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

102000

Methanobacterium formicicum

-

smallest active species, gel filtration

395093

105000

Methanococcus voltae

-

smallest active species, gel filtration, sucrose density centrifugation

395088

109000

Methanobacterium formicicum

-

gel filtration

395093

115000

2 entries

150000

Methanobacterium formicicum

-

gel filtration

395082

170000

2 entries

198000

2 entries

26000

Methanothermobacter thermautotrophicus

-

alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE

395084, 395086

30000

2 entries

30700

Methanospirillum hungatei

-

alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy

395087

31000

Methanothermobacter thermautotrophicus

-

alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE

395084, 395086

33000

2 entries

340000

Methanococcus vannielii

-

gel filtration, enzyme tends to aggregate, aggregated form MW 1300000

395079

380000

Methanobacterium formicicum

-

gel filtration

395082

47000

Methanothermobacter thermautotrophicus

-

alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE

395084, 395086

48000

Methanosarcina barkeri

P80490; P80489; P80491

2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma)

395099

500000

Methanothermobacter thermautotrophicus

-

native enzyme bigger than 500000

395080

50700

Methanospirillum hungatei

-

alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy

395087

600000

Methanobacterium formicicum

-

gel filtration

395082

670000

Methanothermobacter thermautotrophicus

-

membrane-associated protein complex, native PAGE

673769

720000

Methanospirillum hungatei

-

-

395087

745000

Methanococcus voltae

-

gel filtration, sucrose density centrifugation

395088

775000

Methanothermobacter thermautotrophicus

-

gel filtration

395084

790000

2 entries

8000

Methanosarcina barkeri

P80490; P80489; P80491

x * 8000 + x * 33000 + x * 30000, SDS-PAGE

727399

800000

2 entries

845000

Methanosarcina barkeri

P80490; P80489; P80491

non-denaturing PAGE, large enzyme form

727399

960000

Methanothermobacter thermautotrophicus

-

gel filtration

395087

990000

Methanocaldococcus jannaschii

-

native SDS-PAGE

395095

115000

Methanothermobacter thermautotrophicus

-

smallest species with enzymatic activity, native SDS-PAGE

395084, 395086

115000

Methanocaldococcus jannaschii

-

smallest species with enzymatic activity, native SDS-PAGE

395095

170000

Methanothermobacter thermautotrophicus

-

-

395081

170000

Methanobacterium formicicum

-

gel filtration

395083

198000

Methanosarcina barkeri

P80490; P80489; P80491

-

395099

198000

Methanosarcina barkeri

P80490; P80489; P80491

non-denaturing PAGE, small enzyme form

727399

30000

Methanosarcina barkeri

P80490; P80489; P80491

2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma)

395099

30000

Methanosarcina barkeri

P80490; P80489; P80491

x * 8000 + x * 33000 + x * 30000, SDS-PAGE

727399

33000

Methanosarcina barkeri

P80490; P80489; P80491

2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma)

395099

33000

Methanosarcina barkeri

P80490; P80489; P80491

x * 8000 + x * 33000 + x * 30000, SDS-PAGE

727399

790000

Methanothermobacter thermautotrophicus

-

native SDS-PAGE, sucrose density centrifugation

395098

790000

Methanobacterium formicicum

-

biggest enzyme species, aggregate

395085

800000

Methanothermobacter thermautotrophicus

-

gel filtration, native SDS-PAGE

395086

800000

Methanothermobacter thermautotrophicus

-

electron microscopy, shadow casting experiments, ring-shape structure found

395084