1.12.7.2: ferredoxin hydrogenase

This is an abbreviated version!
For detailed information about ferredoxin hydrogenase, go to the full flat file.

Reaction

Synonyms

bidirectional hydrogenase, C-MBH, CooH, CpI, DDH, DdHydAB, EC 1.12.1.1, EC 1.12.7.1, EC 1.18.3.1, EC 1.18.99.1, EC 1.98.1.1, Ech hydrogenase, EHb, EhbM, EhbN, energy-conserving hydrogenase B, energy-converting hydrogenase, Fe hydrogenlyase, Fe-Fe hydrogenase, Fe-H2ase, Fe-hydrogenase, ferredoxin-dependent [FeFe] hydrogenase, ferredoxin-dependent [FeFe]-hydrogenase, H2 oxidizing hydrogenase, H2 producing hydrogenase [ambiguous], HYD1, HYD2, hydA, HydA1, HydA2, hydrogen-lyase [ambiguous], hydrogenase (ferredoxin), hydrogenase I, hydrogenase II, hydrogenase of the Fe-only type, hydrogenlyase, hydrogenlyase [ambiguous], Mbh, MBH hydrogenase, MbhJ, MbhJKLN, MbhK, MbhL, MDH, membrane bound NiFe hydrogenase, membrane-bound hydrogenase, NiFe hydrogenase, reversible Fe-hydrogenase, uptake hydrogenase [ambiguous], [FeFe] hydrogenase, [FeFe] hydrogenase HydA1, [FeFe]-hydrogenase, [Fe]-hydrogenase, [Ni-Fe] hydrogenase, [NiFeSe] Hase, [NiFeSe] hydrogenase

ECTree

     1 Oxidoreductases

         1.12 Acting on hydrogen as donor

             1.12.7 With an iron-sulfur protein as acceptor

                1.12.7.2 ferredoxin hydrogenase

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Results	in table
7	Activating Compound
1868	AA Sequence
3	Application
1	CAS Registry Number
6	Cloned(Commentary)
28	Cofactor
2	Crystallization (Commentary)
3	Expression
10	General Information
3	General Stability
36	Inhibitors
47	KM Value [mM]
28	Localization
67	Metals/Ions
69	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
214	Organism
8	Oxidation Stability
7	Pathway
105	PDB ID
16	pH Optimum
1	pH Range
5	pI Value
14	Protein Variants
31	Purification (Commentary)
1	Reaction
3	Reaction Type
104	Reference
47	Specific Activity [micromol/min/mg]
6	Storage Stability
212	Substrates and Products (Substrate)
33	Subunits
107	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Temperature Stability [°C]
4	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.12.7.2 - ferredoxin hydrogenase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

(SeO3)2- + H2 + ?

Se + H2O + ?

2 entries

(TeO3)2- + H2 + ?

Te + H2O + ?

2 entries

2 H+ + reduced ferredoxin

H2 + oxidized ferredoxin

2 entries

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

6 entries

H+ + NADH

H2 + NAD+

Pyrococcus furiosus

-

-

439641

-

-

r

H+ + reduced benzyl viologen

H2 + oxidized benzyl viologen

Enterobacter cloacae

-

58.8% relative activity, purified enzyme

704088

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

23 entries

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

9 entries

H2 + 2 oxidized ferredoxin

2 reduced ferredoxin + 2 H+

Pyrococcus furiosus

Q8U0Z8; Q8U0Z7; Q8U0Z6

-

730867

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

62 entries

H2 + ferredoxin + oxidized metronidazole

H+ + ferredoxin + reduced metronidazole

2 entries

H2 + methyl viologen

reduced methyl viologen + H+

4 entries

H2 + NAD+

H+ + NADH

Pyrococcus furiosus

-

-

439641

-

-

r

H2 + oxidized benzyl viologen

H+ + reduced benzyl viologen

2 entries

H2 + oxidized benzyl viologen

reduced benzyl viologen + H+

Methanosarcina barkeri

-

wild-type enzyme catalysed the reduction of benzylviologen at fourfold higher rates than the reduction of ferredoxin

673529

-

-

?

H2 + oxidized CAC3527 ferredoxin

H+ + reduced CAC3527 ferredoxin

Clostridium acetobutylicum

-

mutant CAC3527 ferredoxin displays an almost 8fold lower reduction potential than wild type ferredoxin

686267

-

-

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

28 entries

H2 + oxidized ferredoxin

reduced ferredoxin + 2 H+

Chlamydomonas reinhardtii

-

-

705334

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

9 entries

H2 + oxidized ferredoxin CAC0587

H+ + reduced ferredoxin CAC0587

Clostridium acetobutylicum

-

ferredoxin CAC0587 is the standard major ferredoxin

686793

-

-

r

H2 + oxidized ferredoxin Pet F

reduced ferredoxin Pet F + H+

Chlamydomonas reinhardtii

-

-

742279

-

-

?

H2 + oxidized flavodoxin CAC0587

H+ + reduced flavodoxin CAC0587

Clostridium acetobutylicum

-

flavodoxin CAC0587 is the standard major flavodoxin

686793

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

10 entries

H2 + oxidized methylene blue

H+ + reduced methylene blue

2 entries

H2 + oxidized spinach ferredoxin

reduced spinach ferredoxin + H+

2 entries

S + NADPH

H2S + NADP+

2 entries

additional information

?

-

6 entries

(SeO3)2- + H2 + ?

Se + H2O + ?

Clostridium pasteurianum

-

methyl viologen and benzyl viologen as electron acceptors

439661

-

-

?

(SeO3)2- + H2 + ?

Se + H2O + ?

Clostridium pasteurianum W5

-

methyl viologen and benzyl viologen as electron acceptors

439661

-

-

?

(TeO3)2- + H2 + ?

Te + H2O + ?

Clostridium pasteurianum

-

methyl viologen as electron acceptor

439661

-

-

?

(TeO3)2- + H2 + ?

Te + H2O + ?

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439661

-

-

?

2 H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Caldanaerobacter subterraneus subsp. tengcongensis

-

-

675496

-

-

?

2 H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Caldanaerobacter subterraneus subsp. tengcongensis

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Carboxydothermus hydrogenoformans

-

-

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Carboxydothermus hydrogenoformans

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Escherichia coli

-

-

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Escherichia coli

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Rhodospirillum rubrum

-

-

675496

-

-

?

2 H+ + reduced polyferredoxin

H2 + oxidized polyferredoxin

Rhodospirillum rubrum

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Caldanaerobacter subterraneus subsp. tengcongensis

-

-

659110

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Caldanaerobacter subterraneus subsp. tengcongensis

-

-

656734

-

-

r

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Caldanaerobacter subterraneus subsp. tengcongensis

-

reaction is proposed to be coupled to energy conservation

659110

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Chlamydomonas moewusii

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Chlamydomonas reinhardtii

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Chlorococcum submarinum

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Clostridium acetobutylicum

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Clostridium pasteurianum

-

-

439669

-

-

r

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Clostridium pasteurianum

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Desulfovibrio desulfuricans

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Enterobacter cloacae

-

94.3% relative activity, purified enzyme

704088

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Megasphaera elsdenii

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina barkeri

-

-

675496

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina barkeri

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina mazei

-

-

727467

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina mazei

-

Ech hydrogenase acts as primary proton pump in a ferredoxin-dependent electron transport system. Ech hydrogenase is necessary to generate an electrochemical ion gradient when ferredoxin is the only reducing equivalent and heterodisulfide is absent

727467

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina mazei DSM 3647

-

-

727467

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Methanosarcina mazei DSM 3647

-

Ech hydrogenase acts as primary proton pump in a ferredoxin-dependent electron transport system. Ech hydrogenase is necessary to generate an electrochemical ion gradient when ferredoxin is the only reducing equivalent and heterodisulfide is absent

727467

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Pyrococcus furiosus

-

-

659110, 675496

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Pyrococcus furiosus

-

Mbh function as a redox-driven ion pump coupling the reduction of protons with electrons derived from the oxidation of a low-potential ferredoxin to the generation of a H+ motive force

659110

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Pyrococcus furiosus

-

the exergonic reaction is coupled to energy conservation by means of electron-transport phosphorylation

675496

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

Tetradesmus obliquus

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced ferredoxin

H2 + oxidized ferredoxin

[Chlorella] fusca

-

hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin

711817

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Caldanaerobacter subterraneus subsp. tengcongensis

-

-

656734

-

-

r

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Chlamydomonas reinhardtii

-

-

439665, 711817

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Chlamydomonas reinhardtii 137 C(+)

-

-

439665

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Clostridium acetobutylicum

-

-

764411

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Clostridium acetobutylicum NBRC 13948

-

-

764411

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Clostridium pasteurianum

-

-

439669, 439682

-

-

r

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Clostridium pasteurianum W5

-

-

439682

-

-

r

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Enterobacter cloacae

-

100% relative activity, purified enzyme

704088

-

-

?

H+ + reduced methyl viologen

H2 + oxidized methyl viologen

Pyrococcus furiosus

-

-

439641, 659110

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Acidithiobacillus ferrooxidans

-

ferricyanide as electron acceptor

439660

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Acidithiobacillus ferrooxidans

-

methylene blue as electron acceptor

439660

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Acidithiobacillus ferrooxidans ATC 19859

-

ferricyanide as electron acceptor

439660

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Acidithiobacillus ferrooxidans ATC 19859

-

methylene blue as electron acceptor

439660

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii

-

methyl viologen as electron acceptor

439672

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii

-

methyl viologen as electron acceptor

439665

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii

-

sulfonatopropyl viologen, sodium metatungstate and sodium silicotungstate as electron acceptor

439672

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii 137 C(+)

-

methyl viologen as electron acceptor

439665

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii 137 C(+)

-

methyl viologen as electron acceptor

439672

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Chlamydomonas reinhardtii 137 C(+)

-

sulfonatopropyl viologen, sodium metatungstate and sodium silicotungstate as electron acceptor

439672

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

-

439668

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

methylene blue as electron acceptor

439669, 439670, 439679, 439681, 439682, 439684

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

methylene blue as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

methyl viologen as electron acceptor

439669, 439675, 439679, 439681, 439682, 439684

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

methyl viologen as electron acceptor

439671, 439678

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

FAD and FMN as electron acceptors

439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

FAD, FMN or riboflavin as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

benzyl viologen as electron acceptor

439679, 439682

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

benzyl viologen as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum

-

dichlorophenol indophenol as electron acceptor

439679, 439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439669

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439669

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

FAD, FMN or riboflavin as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

benzyl viologen as electron acceptor

439671

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439679

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439679

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

benzyl viologen as electron acceptor

439679

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

dichlorophenol indophenol as electron acceptor

439679

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

FAD and FMN as electron acceptors

439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

dichlorophenol indophenol as electron acceptor

439681

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439682

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439682

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

benzyl viologen as electron acceptor

439682

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methylene blue as electron acceptor

439684

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Clostridium pasteurianum W5

-

methyl viologen as electron acceptor

439684

-

-

?

H2 + electron acceptor

H+ + reduced electron acceptor

Desulfovibrio vulgaris

-

-

439651

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Megasphaera elsdenii

-

methyl viologen as electron acceptor

439674, 439677

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Megasphaera elsdenii

-

benzyl viologen as electron acceptor

439674, 439677

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Megasphaera elsdenii LC1

-

methyl viologen as electron acceptor

439677

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Megasphaera elsdenii LC1

-

benzyl viologen as electron acceptor

439677

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri

-

methylene blue as electron acceptor

439643, 439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri

-

cytochrome b as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri

-

methyl viologen as electron acceptor

439643, 439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri

-

phenosafranine as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri

-

benzyl viologen as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri MS / DSM 800

-

methylene blue as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri MS / DSM 800

-

cytochrome b as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri MS / DSM 800

-

methyl viologen as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri MS / DSM 800

-

phenosafranine as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Methanosarcina barkeri MS / DSM 800

-

benzyl viologen as electron acceptor

439663

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Pyrococcus furiosus

-

methylene blue as electron acceptor

439632

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Pyrococcus furiosus

-

methyl viologen as electron acceptor

439632, 439641, 439652

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Pyrococcus furiosus

-

benzyl viologen as electron acceptor

439641, 439652

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Pyrococcus furiosus

-

NADP+ as electron acceptor

439641, 439652

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Sporomusa sphaeroides

-

cytochrome b as electron acceptor

439659

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Sporomusa sphaeroides

-

methyl viologen as electron acceptor

439659

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Sporomusa sphaeroides

-

benzyl viologen as electron acceptor

439659

-

-

r

H2 + electron acceptor

H+ + reduced electron acceptor

Tetradesmus obliquus

-

methyl viologen as electron acceptor

439650

-

-

r

H2 + ferredoxin + oxidized metronidazole

H+ + ferredoxin + reduced metronidazole

Caldanaerobacter subterraneus subsp. tengcongensis

-

-

656734

-

-

?

H2 + ferredoxin + oxidized metronidazole

H+ + ferredoxin + reduced metronidazole

Methanosarcina barkeri

-

-

673529

-

-

?

H2 + methyl viologen

reduced methyl viologen + H+

Pyrococcus furiosus

-

-

742859

-

-

?

H2 + methyl viologen

reduced methyl viologen + H+

Pyrococcus furiosus COM1

-

-

742859

-

-

?

H2 + methyl viologen

reduced methyl viologen + H+

Rhodospirillum rubrum

-

-

742698

-

-

?

H2 + methyl viologen

reduced methyl viologen + H+

Rhodospirillum rubrum UR1

-

-

742698

-

-

?

H2 + oxidized benzyl viologen

H+ + reduced benzyl viologen

Megasphaera elsdenii

-

-

439674, 439677

-

-

r

H2 + oxidized benzyl viologen

H+ + reduced benzyl viologen

Megasphaera elsdenii LC1

-

-

439677

-

-

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Alvikia littoralis

-

-

439654

-

439654

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Chlamydomonas reinhardtii

-

-

685620, 685627

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Chlamydomonas reinhardtii

-

-

439665

-

439665

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Chlamydomonas reinhardtii

-

-

439672

-

439672

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Chlamydomonas reinhardtii 137 C(+)

-

-

439665

-

439665

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Chlamydomonas reinhardtii 137 C(+)

-

-

439672

-

439672

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium acetobutylicum

-

2[4Fe4S] ferredoxin

686267

-

-

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium acetobutylicum

-

under anoxic conditions

684643

-

-

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum

-

-

439669

-

439669

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum

-

-

439671

-

439671

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum

-

-

439675

-

439675

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum

-

-

439679

-

439679

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum

-

-

439682

-

439682

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum W5

-

-

439669

-

439669

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum W5

-

-

439671

-

439671

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum W5

-

-

439679

-

439679

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Clostridium pasteurianum W5

-

-

439682

-

439682

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Megasphaera elsdenii

-

-

439677

-

439677

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Megasphaera elsdenii LC1

-

-

439677

-

439677

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Methanococcus maripaludis

-

-

712301

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Methanosarcina barkeri

-

-

439643

-

439643

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Methanosarcina mazei

-

-

712307

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Pyrococcus furiosus

-

-

439632

-

439632

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Tetradesmus obliquus

-

ferredoxin links the enzyme to photosynthesis

439650

-

439650

r

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Tetraselmis sp. KSN-2002

-

-

688848

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Tetraselmis sp. KSN-2002 NCIM 1605

-

-

688848

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Thermotoga maritima

-

-

712281

-

-

?

H2 + oxidized ferredoxin

H+ + reduced ferredoxin

Thermotoga maritima DSM 3109

-

-

712281

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Chlamydomonas reinhardtii

-

-

660222

-

-

r

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Chlamydomonas reinhardtii

-

enzyme activity is induced in the dark under anaerobic growth conditions

660222

-

-

r

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Methanosarcina barkeri

-

-

659110

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Methanosarcina barkeri

-

enzyme is absolutely required for the reduction of CO2 to formylmethanofuran by H2, Ech hydrogenase provides the cell with reduced ferredoxin required as electron donor

659110

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Pyrococcus furiosus

-

-

742859

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Pyrococcus furiosus COM1

-

-

742859

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Rhodospirillum rubrum

-

-

742698

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Rhodospirillum rubrum UR1

-

-

742698

-

-

?

H2 + oxidized ferredoxin

reduced ferredoxin + H+

Ruminococcus albus

-

-

742784

-

-

?

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Alvikia littoralis

-

-

439654

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Clostridium acetobutylicum

-

-

684643, 686267, 686793

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Clostridium pasteurianum

-

-

439669

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Clostridium pasteurianum W5

-

-

439669

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Megasphaera elsdenii

-

-

439677

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Megasphaera elsdenii LC1

-

-

439677

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Methanococcus maripaludis

-

-

712301

-

-

?

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Tetradesmus obliquus

-

-

439650

-

-

r

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Tetraselmis sp. KSN-2002

-

-

688848

-

-

?

H2 + oxidized methyl viologen

H+ + reduced methyl viologen

Tetraselmis sp. KSN-2002 NCIM 1605

-

-

688848

-

-

?

H2 + oxidized methylene blue

H+ + reduced methylene blue

Clostridium pasteurianum

-

-

439669

-

-

r

H2 + oxidized methylene blue

H+ + reduced methylene blue

Clostridium pasteurianum W5

-

-

439669

-

-

r

H2 + oxidized spinach ferredoxin

reduced spinach ferredoxin + H+

Rhodospirillum rubrum

-

-

742698

-

-

?

H2 + oxidized spinach ferredoxin

reduced spinach ferredoxin + H+

Rhodospirillum rubrum UR1

-

-

742698

-

-

?

S + NADPH

H2S + NADP+

Pyrococcus furiosus

-

-

439641

-

439641

?

S + NADPH

H2S + NADP+

Pyrococcus furiosus

-

-

439664

-

-

?

additional information

?

-

Desulfovibrio desulfuricans

-

electrodes modified with [FeFe] hydrogenase from Desulfovibrio desulfuricans (DdHydAB) catalyse both H2 oxidation and production under conditions of direct electron transfer or mediated electron transfer within the film of polymer PV2. Reversibility in electrocatalytic films is important for energy conversion applications because it combines the advantage of high current densities at low overpotential with the possibility for robust catalysis owing to the intrinsic protecting feature of the redox matrix

765440

-

-

-

additional information

?

-

Enterobacter cloacae

-

no activity of the purified enzyme with methylene blue, NADH, NADPH and Na-dithionite as electron-donor

704088

-

-

?

additional information

?

-

Methanosarcina barkeri

-

major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells

439643

-

-

?

additional information

?

-

Methanosarcina barkeri Fusaro / DSM 804

-

major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells

439643

-

-

?

additional information

?

-

Pyrococcus furiosus

-

ferredoxin is not an efficient electron carrier for both hydrogenases

439641

-

-

?

additional information

?

-

Pyrococcus furiosus

-

ferredoxin is not an efficient electron carrier for both hydrogenases

439652

-

-

?