relatively stable at 20-30°C, a decrease in POD activity of 75%, 85%, and 90% is found after 10 min at 50°C, 60°C, and 70°C, respectively. The enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min, respectively.
soluble POX2 isoenzyme retains full activity for at least 6 weeks and loses 50% of its activity after 2 months. The immobilized enzyme retains complete activity after 2 months of storage
the enzyme is considerably more stable at 30°C and 40°C where it retains more than 90% of its activity, the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h
the pyromellitic dianhydride-modified enzyme retains 100% activity at 40°C after 15 min, while the native enzyme loses 15% activity. At 80°C, the native enzyme loses 95% activity, while at the same time, the pyromellitic dianhydride-modified enzyme is able to retain 25% activity. The native enzyme retains 75% activity at 50°C for 15 min
the melting temperature is at 70°C for the native enzyme and at 75.4°C for the cofactor-modified enzyme rHRP1 and at 76.5°C for the cofactor-modified enzyme rHRP2. the reconstituted HRPs with modified hemin show higher thermostability in aqueous buffer. After the exposure for 1.5 h at 65°C, native HRP retains only about 15% activity, the reconstituted HRPs, however, retained about 60% activity
protein melting temperature of mutant enzymes T213A and T214A is 88.9°C, protein melting temperature of mutant enzyme T213V is 89.4°C, protein melting temperature of mutant enzyme T213S is 88.5°C
relatively stable between 20-30°C, activity decreases by 20% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min
relatively stable between 20-30°C, activity decreases by 25% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min
30 min, the soluble enzyme retains approximately 13.6% of its activity, the immobilized enzyme entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material retains approximately 32% of its activity
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions
protein melting curves indicate that the thermal stability of CYP119 does not depend on the iron spin state or the active site architecture defined by the threonine residues