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1.11.1.7: peroxidase

This is an abbreviated version!
For detailed information about peroxidase, go to the full flat file.

Word Map on EC 1.11.1.7

Reaction

2 phenolic donor +

H2O2
= 2 phenoxyl radical of the donor + 2 H2O

Synonyms

acidic peroxidase, acidic POD, ALSP, AnaPX, anionic isoperoxidase, anionic peroxidase A1, AOPTP, basic peroxidase, basic POD, BCcP, black radish peroxidase A, black radish peroxidase B, BRP-A, BRP-B, cationic peroxidase, cationic peroxidase Cs, class III plant peroxidase, CmMnP, constitutive peroxidase, CP, cPOD-I, CYP119, dehaloperoxidase A, dehaloperoxidase B, DHP A, donor: H2O2 oxidoreductase, donor: hydrogen peroxide oxidoreductase, donor:hydrogen peroxide oxidoreductase, DtpA, ECPOX, ECPOX 1, ECPOX 2, ECPOX 3, ELP, eosinophil peroxidase, EPO, extensin peroxidase, extracellular peroxidase, FP1, Fp2, FP3, GCP1, GCP2, guaiacol peroxidase, H2O2 oxidoreductase, Hb peroxidase, Hb1, heme peroxidase, hemoglobin 1, hemoglobin peroxidase, hexacoordinate (class 1) non-symbiotic hemoglobin, horseradisch peroxidase, horseradish peroxidase, horseradish peroxidase (HRP), horseradish peroxidase C, HP, HRP, HRP A2, HRP C1A, HRP-C, HRPC, HRPO, HRP_A2A, HRP_C1A, HRP_E5, HTHP, hydrogen donor oxidoreductase, Japanese radish peroxidase, lactoperoxidase, LPO, LPRX, LPS, MAP-2744c, MGP, MnP124076, MnP13, More, MPO, myeloperoxidase, neutral peroxidase, neutral POD, NGO_0994, nsHb-1, oxyperoxidase, p20, PA1, PerII, peroxidase, peroxidase isoenzyme E5, POC1, POD, POD1, POII, POX, POX I, POX II, POX2, protoheme peroxidase, Prx02, Prx03, Prx06, Prx07, Prx09, Prx1, Prx11, Prx114, Prx12, Prx13, Prx15, Prx17, Prx21, Prx22, Prx27, Prx28, Prx30, Prx31, Prx32, Prx33, Prx34, Prx36, Prx37, Prx39, Prx42, Prx43, Prx45, Prx49, Prx50, Prx51, Prx52, Prx53, Prx55, Prx56, Prx57, Prx58, Prx59, Prx60, Prx62, Prx64, Prx66, Prx67, Prx68, Prx69, Prx70, Prx71, Prx72, Prx73, pyrocatechol peroxidase, QPO, quinol peroxidase, rhEPO, rHRP1, rHRP2, royal palm tree peroxidase, rPOD-II, RPTP, rubrerythrin, SacD, Saci_2081, SBP, scavengase, scopoletin peroxidase, SfmD, short PMnP, SPC4, Ssp, stigma-specific peroxidase, thiocyanate peroxidase, thiol peroxidase, TOP, TP I, Tpx, tyrosine-coordinated heme protein, vacuolar class III peroxidase, vascular peroxidase 1, verdoperoxidase, versatile peroxidase, versatile peroxidase VPL2, VP, VPO1, VPO2, WPTP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.7 peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.7 - peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 30
-
isoenzyme POX I is stable
10 - 70
-
stable at 10-40°C and unstable above 50°C, 70-80% of POII activity is lost at 50, 60, and 70°C after 15, 10, and 5 min of incubation, respectively
20
-
isoenzyme POX II is stable
20 - 70
relatively stable at 20-30°C, a decrease in POD activity of 75%, 85%, and 90% is found after 10 min at 50°C, 60°C, and 70°C, respectively. The enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min, respectively.
20 - 80
25
-
soluble POX2 isoenzyme retains full activity for at least 6 weeks and loses 50% of its activity after 2 months. The immobilized enzyme retains complete activity after 2 months of storage
30 - 60
-
the enzyme is considerably more stable at 30°C and 40°C where it retains more than 90% of its activity, the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h
40 - 50
40 - 80
-
the pyromellitic dianhydride-modified enzyme retains 100% activity at 40°C after 15 min, while the native enzyme loses 15% activity. At 80°C, the native enzyme loses 95% activity, while at the same time, the pyromellitic dianhydride-modified enzyme is able to retain 25% activity. The native enzyme retains 75% activity at 50°C for 15 min
5 - 85
the enzyme shows high stability between 5 and 65°C, but is inactivated after drastic heat treatment (10 min at 85°C)
52
-
no loss of activity after 20 min
58
-
no loss of activity after 20 min
60 - 80
-
the enzyme remains stable at 60°C for 60 min, POD has no activity above 80°C
62
-
no loss of activity after 20 min
65 - 76.5
-
the melting temperature is at 70°C for the native enzyme and at 75.4°C for the cofactor-modified enzyme rHRP1 and at 76.5°C for the cofactor-modified enzyme rHRP2. the reconstituted HRPs with modified hemin show higher thermostability in aqueous buffer. After the exposure for 1.5 h at 65°C, native HRP retains only about 15% activity, the reconstituted HRPs, however, retained about 60% activity
65.2
-
approx. 50% loss of activity after 20 min, complete loss of activity after 90 min
66.5
-
approx. 50% loss of activity after 15 min, complete loss of activity after 70 min
67
Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C
70 - 100
-
activity decreases at 70°C and above
71
-
approx. 50% loss of activity after 3 min, complete loss of activity after 10 min
82
Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C
87
protein melting temperature of mutant enzyme T213A/T214A is 87.0°C
88
protein melting temperature of mutant enzymes T213F and T213W is 88.1°C
89
protein melting temperature of mutant enzymes T213A and T214A is 88.9°C, protein melting temperature of mutant enzyme T213V is 89.4°C, protein melting temperature of mutant enzyme T213S is 88.5°C
90
-
temperature required for total inactivation
91
protein melting temperature of wild-type enzyme is 90.5°C
93
protein melting temperature of mutant enzyme T214V is 92.8°C
additional information