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1.11.1.6: catalase

This is an abbreviated version!
For detailed information about catalase, go to the full flat file.

Word Map on EC 1.11.1.6

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

Ab-catalase, BNC, caperase, CAT, CAT-1, CAT-A, CAT-P, Cat1.4, CatA, catalase, catalase A, catalase C, catalase form III, catalase P, catalase-1, catalase-A, catalase-peroxidase, catalase-phenol oxidase, CatB, CATC, CatF, CatG, CatP, CATPO, CcmC, CP, equilase, H2O2:H2O2 oxidoreductase, haem catalase, HPI-A, HPI-B, HPII, HTHP, hydrogen peroxide oxidoreductase, KAT, Kat E catalase, KatA, KatB, KatC, KatP, KpA, manganese catalase, More, optidase, PktA, polyethylene glycol-catalase, tyrosine-coordinated heme protein, VktA

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.6 catalase

Purification

Purification on EC 1.11.1.6 - catalase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3-step procedure, 65fold
-
3.8fold from cell culture supernatant
-
91fold from crude cell extract
acetone precipitation and Sephadex G-150 gel filtration
active native enzyme by ultracentrifugation, streptomycin sulfate treatment, by drophobic interaction, anion exchange, and hydroxyapatite chromatography, gel filtration, followed by another step of anion exchange chromatography
ammonium sulfate precipitation, DEAE column chromatography, and Sephacryl Tm S-200 gel filtration
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, and Sephacryl gel filtration
-
catalase-peroxidase enzyme to homogeneity, catalase enzyme partial
-
development of simple methods for production and purification of catalases, mineral sorbents binding the enzyme from solution, overview
-
enzyme from a Xanthomonas campestris strain
from culture broth of cells grown on ethanol
-
isoforms Cat-1, Cat-2, Cat-3
native enzyme 106fold from seedlings by ammonium sulfate fractionation and immobilized metal affinity chromatography, best results by using Zn2+ affinity
-
native enzyme 11fold by anion exchange and hydrophobic chromatography, followed by ultrafiltration
native enzyme from cytoplasm and cell envelope
-
native enzyme from the soluble fraction, 47.3fold to homogeneity
-
near homogeneity, 3 isoenzymes
-
near homogeneity, chromatography techniques
partial
partial, recombinant isoenzyme
-
purified to homogeneity as holoprotein by affinity chromatography
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography
-
recombinanat wild-type and mutant His-tagged Cat1.4 isozymes from Escherichia coli strain BL21 (DE3) to homogeneity
-
recombinant enzyme
recombinant enzyme from membranes by solubilization with beta--dodecylmaltoside, immuno-affinity chromatography using a Strep-tagged antibody, and ultrafiltration. X-ray crystallography as well as mass spectrometry analysis reveal no signs for any covalent modification of subunit I besides the crosslink between H276 and Y280
recombinant His-tagged and wild-type enzymes from Bacillus subtilis strain BLF03, 195fold, and Enterococcus faecalis strain V583
-
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography
-
recombinant mutant M244A
-
recombinant protein
recombinant soluble and inactive His-tagged catalase-A from Escherichia coli strain BL21(DE3) inclusion bodies by EK-affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) to homogeneity by anion exchange chromatography and gel filtration
-
to homogeneity by ammonium sulfate fractionation and several chromatographic steps
-
to homogeneity, chromatography steps
to homogeneity, chromatography techniques
to homogeneity, precipitation and chromatography steps
-
to homogeneity, precipitation and chromatography techniques
to homogeneity, three isoforms
-
Triton X-100 supplementation can markedly improve extracellular ratio of KatA
TvC-II, 300fold to homogeneity, TvC-I partially purified, TvC-I separation from TvC-II
-