1.11.1.16: versatile peroxidase
This is an abbreviated version!
For detailed information about versatile peroxidase, go to the full flat file.
Word Map on EC 1.11.1.16
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1.11.1.16
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lignin
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pleurotus
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peroxidases
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ligninolytic
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eryngii
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laccase
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white-rot
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bjerkandera
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ostreatus
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veratryl
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adusta
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chrysosporium
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phanerochaete
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lignin-degrading
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non-phenolic
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2,6-dimethoxyphenol
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delignification
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aryl-alcohols
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degradation
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synthesis
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industry
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analysis
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paper production
- 1.11.1.16
- lignin
- pleurotus
- peroxidases
-
ligninolytic
- eryngii
- laccase
-
white-rot
- bjerkandera
- ostreatus
-
veratryl
- adusta
- chrysosporium
-
phanerochaete
-
lignin-degrading
-
non-phenolic
- 2,6-dimethoxyphenol
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delignification
-
aryl-alcohols
- degradation
- synthesis
- industry
- analysis
- paper production
Reaction
Synonyms
B-type dye-decolorizing peroxidase, bacterial lignin peroxidase, DypB, manganese peroxidase 4, Mb peroxidase, metMb peroxidase, Mnp4, More, myoglobin, R1B4, versatile peroxidase, versatile peroxidase MnP2, versatile peroxidase VPL2 precursor, VP1, Vpl2, VPS1
ECTree
Advanced search results
KM Value
KM Value on EC 1.11.1.16 - versatile peroxidase
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6.4
manganese(II)-substituted polyoxometalate
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in 0.1 M sodium tartrate, pH 5.0, at 20°C
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0.0007
pH 3.5, 25°C
0.0017
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant R257L
0.0023
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant P76G, high efficiency site, pH 3.5, 25°C
0.0026
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant M247L
0.0028
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant S158E
0.003
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, wild-type
0.003
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type, high efficiency site, pH 3.5, 25°C
0.0032
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant R257A/A260F
0.0035
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant M247F
0.0036
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant R257K
0.004
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant S158D
0.004
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K176D, high efficiency site, pH 3.5, 25°C
0.0041
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant F142G, high efficiency site, pH 3.5, 25°C
0.0054
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K176G, high efficiency site, pH 3.5, 25°C
0.0054
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K215G, high efficiency site, pH 3.5, 25°C
0.0054
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant P141G, high efficiency site, pH 3.5, 25°C
0.0065
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant K264A
0.008
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K215Q, high efficiency site, pH 3.5, 25°C
0.009
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 25°C, mutant A260F
0.0143
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant E140G, high efficiency site, pH 3.5, 25°C
0.0194
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant E140G/P141G, high efficiency site, pH 3.5, 25°C
0.0194
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant E140G/P141G, low efficiency site, pH 3.5, 25°C
0.056
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication
0.0836
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K215G, low efficiency site, pH 3.5, 25°C
0.18
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
at 30°C and pH 4.5
0.305
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant E140G, low efficiency site, pH 3.5, 25°C
0.383
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K176G, low efficiency site, pH 3.5, 25°C
0.461
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant P141G, low efficiency site, pH 3.5, 25°C
0.48
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
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absence of Mn2+, pH 3.0, temperature not specified in the publication
0.54
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type, pH 3.5, temperature not specified in the publication
0.54
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
at pH 3.0 and 25°C
0.828
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant F142G, low efficiency site, pH 3.5, 25°C
0.86
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
presence of Mn2+, pH 3.0, temperature not specified in the publication
1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant W164S, low efficiency site, pH 3.5, 25°C
1.09
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type, low efficiency site, pH 3.5, 25°C
1.66
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K215Q, low efficiency site, pH 3.5, 25°C
2.23
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant K176D, low efficiency site, pH 3.5, 25°C
2.86
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant P76G, low efficiency site, pH 3.5, 25°C
14.9
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 1.5 mM H2O2
15.6
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 0.3 mM H2O2
0.036
2,6-dimethoxyphenol
mutant P76G, high efficiency site, pH 3.5, 25°C
0.038
2,6-dimethoxyphenol
mutant K176D, high efficiency site, pH 3.5, 25°C
0.058
2,6-dimethoxyphenol
mutant K215G, high efficiency site, pH 3.5, 25°C
0.063
2,6-dimethoxyphenol
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presence of Mn2+, pH 7.0, temperature not specified in the publication
0.065
2,6-dimethoxyphenol
mutant F142G, high efficiency site, pH 3.5, 25°C
0.066
2,6-dimethoxyphenol
mutant E140G, high efficiency site, pH 3.5, 25°C
0.078
2,6-dimethoxyphenol
mutant K215Q, high efficiency site, pH 3.5, 25°C
0.078
2,6-dimethoxyphenol
wild-type, high efficiency site, pH 3.5, 25°C
0.1
2,6-dimethoxyphenol
mutant K176G, high efficiency site, pH 3.5, 25°C
0.104
2,6-dimethoxyphenol
mutant E140G/P141G, high efficiency site, pH 3.5, 25°C
0.119
2,6-dimethoxyphenol
mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C
0.155
2,6-dimethoxyphenol
mutant P141G, high efficiency site, pH 3.5, 25°C
0.189
2,6-dimethoxyphenol
mutant E140G/K176G, high efficiency site, pH 3.5, 25°C
0.345
2,6-dimethoxyphenol
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absence of Mn2+, pH not specified in the publication, temperature not specified in the publication
2.38
2,6-dimethoxyphenol
mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C
2.56
2,6-dimethoxyphenol
mutant K215Q, low efficiency site, pH 3.5, 25°C
2.97
2,6-dimethoxyphenol
mutant E140G/K176G, low efficiency site, pH 3.5, 25°C
3.33
2,6-dimethoxyphenol
mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C
6.5
2,6-dimethoxyphenol
mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication
10.5
2,6-dimethoxyphenol
wild-type, low efficiency site, pH 3.5, 25°C
16
2,6-dimethoxyphenol
mutant E140G/P141G, low efficiency site, pH 3.5, 25°C
17.5
2,6-dimethoxyphenol
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 0.3 mM H2O2
29.5
2,6-dimethoxyphenol
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 1.5 mM H2O2
32
2,6-dimethoxyphenol
wild-type, pH 3.5, temperature not specified in the publication
36.2
2,6-dimethoxyphenol
mutant K176G, low efficiency site, pH 3.5, 25°C
37.4
2,6-dimethoxyphenol
mutant E140G, low efficiency site, pH 3.5, 25°C
37.6
2,6-dimethoxyphenol
mutant W164S, low efficiency site, pH 3.5, 25°C
76
2,6-dimethoxyphenol
mutant P141G, low efficiency site, pH 3.5, 25°C
0.009
-
absence of Mn2+, pH 4.5, temperature not specified in the publication
0.038
2,7-diaminofluorene
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presence of Mn2+, pH not specified in the publication, temperature not specified in the publication
0.0059
mutant F142G, high efficiency site, pH 3.5, 25°C
0.0103
4-hydroquinone
mutant P76G, high efficiency site, pH 3.5, 25°C
0.0122
4-hydroquinone
mutant K215Q, high efficiency site, pH 3.5, 25°C
0.013
4-hydroquinone
mutant K176D, high efficiency site, pH 3.5, 25°C
0.0174
4-hydroquinone
mutant K215G, high efficiency site, pH 3.5, 25°C
0.0189
4-hydroquinone
mutant K176G, high efficiency site, pH 3.5, 25°C
0.0205
4-hydroquinone
mutant E140G, high efficiency site, pH 3.5, 25°C
0.0251
4-hydroquinone
mutant E140G/K176G, high efficiency site, pH 3.5, 25°C
0.0362
4-hydroquinone
mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C
0.0397
4-hydroquinone
mutant E140G/P141G, high efficiency site, pH 3.5, 25°C
0.04
4-hydroquinone
mutant P141G, high efficiency site, pH 3.5, 25°C
0.41
4-hydroquinone
mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C
0.618
4-hydroquinone
mutant E140G/K176G, low efficiency site, pH 3.5, 25°C
0.836
4-hydroquinone
mutant E140G, low efficiency site, pH 3.5, 25°C
0.884
4-hydroquinone
mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C
1.112
4-hydroquinone
mutant K215G, low efficiency site, pH 3.5, 25°C
1.114
4-hydroquinone
mutant P141G, low efficiency site, pH 3.5, 25°C
1.18
4-hydroquinone
mutant E140G/P141G, low efficiency site, pH 3.5, 25°C
0.03
H2O2
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 50 mM 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
0.04
H2O2
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 50 mM 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2 mM Mn2+
0.051
H2O2
wild-type, pH 3.5, temperature not specified in the publication
0.2
H2O2
mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication
3.1
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 0.3 mM H2O2
11.2
Mn(II)
pH 5.0, 25°C, recombinant enzyme mutant N246A, in presence of 1.5 mM H2O2
0.045
Mn2+
wild-type, pH 3.5, temperature not specified in the publication
0.12
Mn2+
mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication
0.68
Mn2+
-
pH not specified in the publication, temperature not specified in the publication
0.0066
Reactive Black 5
mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication
0.007
Reactive Black 5
wild-type, pH 3.5, temperature not specified in the publication
0.035
-
presence of Mn2+, pH 7.0, temperature not specified in the publication
0.066
syringaldazine
-
absence of Mn2+, pH 7.0, temperature not specified in the publication
0.304
veratryl alcohol
-
absence of Mn2+, pH 3.0, temperature not specified in the publication
0.53
veratryl alcohol
-
presence of Mn2+, pH 4.5, temperature not specified in the publication
additional information
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Hill coefficient 3.0, presence of Mn2+, Km value 0.009 g/l, pH 3.5, 25°C
additional information
fulvic acid
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Hill coefficient 3.6, absence of Mn2+, Km value 0.044 g/l, pH 3.5, 25°C
additional information
humic acid
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Hill coefficient 1.2, absence of Mn2+, Km value 0.08 g/l, pH 3.5, 25°C
additional information
humic acid
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Hill coefficient 2.8, presence of Mn2+, Km value 0.005 g/l, pH 3.5, 25°C
additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
kinetic parameters for wild-type enzyme and five selected mutant variants with dye substrates, comparisons, overview
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additional information
additional information
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kinetic parameters for wild-type enzyme and five selected mutant variants with dye substrates, comparisons, overview
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additional information
additional information
Michaelis-Menten kinetics by mutant enzyme N246A
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additional information
additional information
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Michaelis-Menten steady-state kinetic analysis, recombinant enzyme
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