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1.11.1.14: lignin peroxidase

This is an abbreviated version!
For detailed information about lignin peroxidase, go to the full flat file.

Word Map on EC 1.11.1.14

Reaction

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
3,4-dimethoxybenzaldehyde
+
2-methoxyphenol
+
glycolaldehyde
+
H2O

Synonyms

ALiP-P3, bacterial lignin peroxidase, diarylpropane oxygenase, diarylpropane peroxidase, diarylpropane:oxygen,hydrogen-peroxide oxidoreductase (C-C-bond-cleaving), DypB, fungal lignin peroxidase, Glg4, H2O2-dependent ligninase, heme-containing lignin peroxidase, heme-containing peroxidase, lignin peroxidase, lignin peroxidase H8, lignin peroxidase isozyme H8, lignin peroxidase LIII, ligninase, ligninase H2, ligninase H8, ligninase I, ligninase LG5, LIP, Lip1, LIP2, LiPH8, lipJ, LPA, LPOA, microbial lignin peroxidase, More, mushroom tyrosinase, oxygenase, diarylpropane, Pr-lip1, Pr-lip4

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.14 lignin peroxidase

Cloned

Cloned on EC 1.11.1.14 - lignin peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
design of a synthetic gene encoding the N246A variant of DypB from Rhodococcus jostii strain RHA1 (Rh_DypB) is designed by back translation of the protein sequence, recombinant overexpression of His-tagged N246A mutant enzyme in Escherichia coli strain BL21(DE3), the enzyme is fully produced as folded holoenzyme, thus without the need for a further reconstitution step
enzyme cloning from strain NK-1, phylogenetic analysis
-
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21(DE3)pLysS cells, recombinant LiP protein accumulates in inclusion bodies as an inactive form
expression in Escherichia coli
expression in Saccharomyces cerevisiae
-
gene LPOA, recombinant expression of the codon-optimized gene in Escherichia coli strain W3110. The cloned expression vector pFLAG1 and the resulting plasmid pFLAG1-Y00262 are directly used for expression, Escherichia coli strain DH5x02alpha is used for plasmid propagation. The apoenzyme accumulates in inclusion bodies
homologous overexpression in Phanerochaete sordida YK-624 uracil auxotrophic mutant UV-64
overexpression of isozyme H8 and W171 mutant in Escherichia coli
-