1.11.1.13: manganese peroxidase
This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.
Word Map on EC 1.11.1.13
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1.11.1.13
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lignin
-
laccase
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ligninolytic
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phanerochaete
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chrysosporium
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white-rot
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basidiomycete
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decolor
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peroxidases
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pleurotus
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lignin-degrading
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trametes
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versicolor
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veratryl
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ostreatus
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straw
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mniii
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lignocellulosic
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bjerkandera
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sordida
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lignocellulolytic
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remazol
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subvermispora
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lacteus
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eryngii
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irpex
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lignin-modifying
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kraft
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sawdust
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phlebia
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adusta
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ceriporiopsis
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1-hydroxybenzotriazole
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nutrition
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lignocellulose-degrading
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ligninase
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sajor-caju
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wood-rotting
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dye-decolorizing
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tigrinus
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mnso4
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biosorption
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delignification
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delignified
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biobleaching
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degradation
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synthesis
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lentinula
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biotreatment
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non-phenolic
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industry
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decolourisation
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2,6-dimethoxyphenol
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paper production
-
low-nitrogen
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environmental protection
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biotechnology
-
biofuel production
- 1.11.1.13
- lignin
- laccase
-
ligninolytic
- phanerochaete
- chrysosporium
-
white-rot
-
basidiomycete
-
decolor
- peroxidases
- pleurotus
-
lignin-degrading
- trametes
- versicolor
-
veratryl
- ostreatus
- straw
-
mniii
-
lignocellulosic
- bjerkandera
- sordida
-
lignocellulolytic
-
remazol
- subvermispora
- lacteus
- eryngii
- irpex
-
lignin-modifying
-
kraft
- sawdust
- phlebia
- adusta
-
ceriporiopsis
- 1-hydroxybenzotriazole
- nutrition
-
lignocellulose-degrading
-
ligninase
- sajor-caju
-
wood-rotting
-
dye-decolorizing
- tigrinus
- mnso4
-
biosorption
-
delignification
-
delignified
-
biobleaching
- degradation
- synthesis
- lentinula
-
biotreatment
-
non-phenolic
- industry
-
decolourisation
- 2,6-dimethoxyphenol
- paper production
-
low-nitrogen
- environmental protection
- biotechnology
- biofuel production
Reaction
2 Mn(II)
+
2 H+
+
Synonyms
CmMnP, extralong PMnP, hybrid Mn-peroxidase, Il-MnP1, Il-MnP6, L-MnP, LeMnP2, long PMnP, manganese peroxidase, manganese peroxidase 2, manganese-dependent peroxidase, MGmn2, MGmnp1, MGmnp3, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:H2O2 oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-BBP6, MnP-GY, MnP-PGY, mnp1, MnP10, MnP117436, MnP12, MnP157986, MnP2, MnP3, MnP50297, MnP6, Moror_3885, MP, MrMnP1, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2, rMnP3-BBP6, short manganese peroxidase, short MnP
ECTree
1.11.1.13 manganese peroxidaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 1.11.1.13 - manganese peroxidase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
100
20 - 55
over 80% of maximal activity remains, recombinant enzyme, temperature stability profile, overview
35
37
-
recombinant MnP: 5 min, 50% loss of activity, A48C/A63C double mutant MnP: relatively stable
40
40 - 60
45
45 - 50
Phlebia sp. MG-60
purified isozyme MGMnP2 is stable up to 45°C while the activity decreases around 50°C and disappears completely at 60°C after 1 h
49
-
wild-type MnP, MnP F190Y and MnP F190L: 330 s, 50% loss of activity, MnP F190I: 30 s, 50% loss of activity, MnP F190A: 5 s, complete loss of activity
50
50 - 70
-
the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively
52
-
recombinant MnP: 20 s, 50% loss of activity, A48C/A63C double mutant MnP: 2 min, 50% loss of activity
55
60
65
additional information
100
-
complete inactivation after 5 min, 20% of original activity recovered after storage of heat-inactivated protein for 1 h at 0°C, no reactivation possible after boiling for 20 min
40 - 60
Phlebia sp. MG-60
stable up to 40°C, while activity decreases above. MGMnP1 keeps half of its activity at 60°C after 1 h
40 - 60
Phlebia sp. MG-60
stable up to 40°C, while activity decreases rapidly above for MGMnP3 after 1 h incubation, inactivation at 50°C
45
purified recombinant refolded His6-tagged enzyme retaining over 90% of its enzymatic activity below 45°C for 12 h
50
-
20% decrease of activity after 20 min, no further loss of activity after 60 min
55
-
more thermostable than MnP from Phanerochaete chrysosporium with half-lives 15-40fold longer at 55°C, Mn2+, Cd2+ and Zn2+ enhances thermostability
55
-
pH 4.5, 1.4 min, 50% loss of activity, both Mn2+ and Cd2+ protect MnP from thermal denaturation more efficiently than Ca2+ at pH 4.5, extending the half-life more than 2fold at 1 mM, combination of 0.5 mM Mn2+ and 0.5 mM Cd2+ extends the half-life more than 10fold
55
-
pH 4.5, 3 min, wild-type enzyme loses about 75% of the initial activity, 50% loss of activity after 3 min in presence of 1 mM ferric chloride. pH 4.5, 2 min, recombinant enzyme loses about 90% of the initial activity, about 45% loss of activity in presence of 1 mM ferric chloride
60
-
80% loss of activity after 10 min, complete inactivation after 40 min
additional information
-
polyethylene glycol-modified enzyme shows greater stability to higher temperatures than native enzyme
additional information
-
Mn2+ protects against thermal inactivation, probably due to active site protection
additional information
very sensitive to thermal treatment
additional information
-
susceptible to thermal inactivation due to the loss of calcium, calcium content after thermal treatment at 37°C for 15 min: 1.5 mol per mol of native MnP, 1 mol per mol of recombinant MnP and 1.3 mol per mol of A48C/A63C double mutant MnP, biphasic inactivation kinetics, recombinant MnP is more sensitive than native MnP as a result of the lack of glycosylation, calcium decreases the rate of thermal inactivation and reactivates MnP up to 50% of its original activity, while EGTA increases the rate of inactivation
additional information
Phlebia sp. MG-60
isozyme MGMnP1 shows relatively high thermotolerance compared with the other isozymes
additional information
Phlebia sp. MG-60
isozyme MGMnP1 shows relatively high thermotolerance compared with the other isozymes
additional information
Phlebia sp. MG-60
isozyme MGMnP1 shows relatively high thermotolerance compared with the other isozymes
additional information
Phlebia sp. MG-60
isozyme MGMnP3 shows relatively low thermotolerance compared with isozyme MGMnP1
additional information
Phlebia sp. MG-60
isozyme MGMnP3 shows relatively low thermotolerance compared with isozyme MGMnP1
additional information
Phlebia sp. MG-60
isozyme MGMnP3 shows relatively low thermotolerance compared with isozyme MGMnP1
