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1.11.1.11: L-ascorbate peroxidase

This is an abbreviated version!
For detailed information about L-ascorbate peroxidase, go to the full flat file.

Word Map on EC 1.11.1.11

Reaction

2 L-ascorbate +

H2O2
+ 2 H+ = 2 monodehydroascorbate + 2 H2O

Synonyms

Am-pAPX1, APEX2, APOX, APX, APX 1, APX 2, APX1, APX2, APX3, APX4, APX5, APX6, APX7, APx8, APXS, APXT, ascorbate peroxidase, ascorbate peroxidase 1, ascorbate peroxidase 2, ascorbate peroxidase 3, ascorbate peroxidase 4, ascorbate peroxidase 5, ascorbate peroxidase 6, ascorbate peroxidase 7, ascorbate peroxidase 8, ascorbate peroxidase6, ascorbic acid peroxidase, At1g07890, AT1G77490, AT3G09640, AT4G08390, AT4G32320, AT4G35000, AT4G35970, AtAPx08, AtAPX1, AtAPX2, AtAPX3, AtAPX5, AtAPX6, AtSAPX, AtstAPX, AtTAPX, cAPX, cAPX 2, CmstAPX, CrAPX4, CreAPX1, CreAPX2, CreAPX4, CreAPXheme, CsAPX1, cytoplasmic ascorbate peroxidase 1, cytosolic ascorbate peroxidase, GhAPX1, glyoxysomal APX, HvAPX1, L-ascorbate peroxidase, L-ascorbate peroxidase 3, L-ascorbate peroxidase 5, L-ascorbate peroxidase 6, L-ascorbate peroxidase, heme-containing, L-ascorbic acid peroxidase, L-ascorbic acid-specific peroxidase, LmAPX, MaAPX1, OsAPx1, OsAPx2, OsAPx3, OsAPx4, OsAPx5, OsAPx6, OsAPx7, OsAPx8, OsAPXa, OsAPXb, pAPX, Pavirv00022559m, peroxidase, ascorbate, peroxisomal ascorbate peroxidase, PgAPX1, PHYPA_001206, PHYPA_001884, PHYPA_021776, PHYPA_024580, PHYPA_024582, Potri.002G081900, Potri.004G174500, Potri.005G112200, Potri.005G161900, Potri.005G179200, Potri.006G089000, Potri.006G132200, Potri.006G254500, Potri.009G015400, Potri.009G134100, Potri.016G084800, PpAPX, PpAPX-S, PpAPX2, PpAPX2.1, PpAPX2.2, PpAPX3, PpAPX6-related, PtAPX-S.1, PtAPX-S.2, PtAPX-TL29, PtAPX.3, PtAPX1.1, PtAPX1.2, PtAPX2, PtAPX3, PtAPX5, PtAPX5-like, PtAPX6 related, PtotAPX, RcAPX, sAPX, stromal APX, stromal ascorbate peroxidase, stromal ascorbate peroxidases, TaAPX, tAPX, thylakoid ascorbate peroxidase, thylakoid membrane-bound ascorbate peroxidases, thylakoid-bound ascorbate peroxidase

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.11 L-ascorbate peroxidase

Purification

Purification on EC 1.11.1.11 - L-ascorbate peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, butyl-Toyopearl column chromatography, DEAE-cellulofine column chromatography, and Sephadex G-75 gel filtration
-
Arabidopsis thaliana leaf extracts are prepared
-
both APX 1 and APX 2
-
Escherichia coli recombinant stromal ascorbate peroxidase is purified with a HisTrap kit, proteins are used to immunize rabbits and rats
mitochondria are prepared and fractionated
-
native enzyme 152fold from leaf juice by ultrafiltration, dialysis, and anion exchange chromatography, to homogeneity
-
of recombinant enzyme 1: using column chromatography on DEAE Bio-Gel A agarose and column chromatography on Sephacryl S-300, of recombinant enzyme 2: using column chromatography on DEAE Bio-Gel A agarose, ammonium sulfate fractionation, dialysis, chromatofocusing on a polybuffer 94 exchanger and column chromatography on Sephacryl S-300
-
of the native enzyme, using ammonium sulfate precipitation, column chromatography on Phenyl-Sepharose, second ammonium sulfate precipitation, and column chromatography on Superdex 200 and Q Sepharose
Chlamydomonas sp.
of the recombinant enzyme, using affinity chromatography on amylose colum, and column chromatography on hydroxylapatite and FFQ-Sepharose
-
partial, from whole body homogenate, using ammonium sulfate fractionation, column chromatography on Sephadex G-75 and isoelectric focusing. Second isolation of the enzyme: from regurgitant of actively feeding fifth instar organism, using centrifugation, a concentrator and isoelectric focusing
-
purified near homogeneity from clarified Escherichia coli lysate by passing through Ni-NTA agarose beads
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant Escherichia coli mutants are purified by chromatography on a Sephadex G-75 column and a DEAE-Sephacel anione-exchange column
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione and nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, ultrafiltration, and gel filtration, again followed by ultrafiltration
TALON metal affinity resin column chromatography
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, and a second ammonium sulfate fractionation
-
using ammonium sulfate precipitation, dialysis and column chromatography on butyl-Toyopearl, DEAE-cellulofine and Sephadex G-75
-
using ammonium sulfate precipitation, dialysis and column chromatography on DEAE, Sephacryl und hydroxyapatite
-
using Ca-phosphate gel treatment, ammonium sulfate fractionation, column chromatography on Sephadex G-100, ultrafiltration and column chromatography on DEAE-Sephadex and CM-Sephadex C-50, two forms of the enzyme, B and C, can be separated by the CM-Sephadex C-50 step
-
using column chromatography on DEAE-Sephacel, ammonium sulfate treatment and column chromatography on butyl-Toyopearl and TSK G3000SW
-
using hydrophobic chromatography, separation of two isozymes: enzyme-A and enzyme-B, enzyme-B accounts for 85% of the total activity, purification of both enzymes
-
using lysed mitochondria, and column chromatography on DEAE-Sephacel, Sephadex G-75, DE-52 and hydroxylapatite
-
using Ni2+-agarose affinity chromatography and gel filtration after reconstitution with hemin
-
using ultra centrifugation, column chromatography on DEAE-Sephacel, ammonium sulfate precipitation, column chromatography on phenyl Sepharose, ammonium sulfate precipitation and column chromatography on Superdex 200 and Phenyl Superose
-
using ultracentrifugation, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-150, DEAE-cellulose and CM-cellulose
-
wild-type and mutant enzyme W208F expressed in Escherichia coli
-