1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

1.10.3.3

copper

dehydroascorbic

electrode

laccase

oxidases

ceruloplasmin

zucchini

cucurbita

cucumber

amperometric

trinuclear

apoplastic

monodehydroascorbate

squash

multi-copper

ascorbate-dependent

copper-containing

plastocyanin

pumpkin

azurins

ascorbyl

ascorbate-glutathione

myrothecium

vernicifera

ferroxidase

agriculture

medicine

analysis

Reaction

4 L-ascorbate +

= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, Af_AO1, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

1 Oxidoreductases

1.10 Acting on diphenols and related substances as donors

1.10.3 With oxygen as acceptor

1.10.3.3 L-ascorbate oxidase

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Results	in table
15	Activating Compound
1056	AA Sequence
11	Application
1	CAS Registry Number
21	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
19	Disease/ Diagnostics
5	Expression
21	General Information
9	General Stability
84	Inhibitors
11	Ki Value [mM]
49	KM Value [mM]
42	Localization
41	Metals/Ions
33	Molecular Weight [Da]
38	Natural Substrates/ Products (Substrates)
134	Organism
1	Oxidation Stability
2	Pathway
8	PDB ID
35	pH Optimum
3	pH Range
6	pH Stability
7	Posttranslational Modification
32	Protein Variants
15	Purification (Commentary)
8	Reaction
3	Reaction Type
128	Reference
2	Renatured (Commentary)
94	Source Tissue
2	Specific Activity [micromol/min/mg]
6	Storage Stability
125	Substrates and Products (Substrate)
28	Subunits
84	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
1	Temperature Range [°C]
26	Temperature Stability [°C]
21	Turnover Number [1/s]

General Stability

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General Stability on EC 1.10.3.3 - L-ascorbate oxidase

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20-25% retention of activity after immobilization, at 12°C, stable for at least 30 days

Cucurbita pepo medullosa

439919

a long acting enzyme derivative is synthesized by covalently linking poly(ethylene glycol) to the enzyme

Cucurbita sp.

696121

ascorbate oxidase is alternately deposited with Au nanoparticles and forms a layer-by-layer membrane on a Pt electrode. The enzyme activities remain even after the deposition. The oxidation current of ascorbic acid (0.1 mM) decreases to 36% for the (ascorbate oxidase/Au)1 modified electrode, and to 19% for the (ascorbate oxidase/Au)10 modified electrode

Cucumis sp.

690102

gelatin, catalase, peroxidase and methemoglobin protect against inactivation

Cucurbita pepo condensa

439889

immobilization within 6% Ca-alginate gel beads improves stability

Cucurbita moschata

439911

no change in total activity in the presence of 5 M urea, conversion of tetrameric form into monomer with 75% activity in 8 M urea

Cucurbita pepo medullosa

439924

partially unfolding of the enzyme by 1.4 M guanidinium hydrochloride or 2.8 M urea

Cucurbita pepo

P37064

673569

role of copper in stability

Cucurbita pepo medullosa

439912

stable to dialysis against EDTA or cyanide

Albifimbria verrucaria

396331