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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Temperature Stability

Temperature Stability on EC 1.10.3.2 - laccase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 25
-
purified recombinant enzyme, stable
0 - 50
-
purified enzyme, stable
10
-
1 h, 5% loss of activity
10 - 30
-
at pH 6, the rate of inactivation increases as the temperature is elevated from 10 to 60°C, with good stability observed in the range of 10 to 30°C
20 - 30
16 h, less than 5% loss of activity
20 - 50
-
purified enzyme, 90 min, completely stable
20 - 70
temperature stability profiles of native and deglycosylated enzymes, overview
20 - 80
stable from 20°C-80°C
22
-
50 h, about 55% loss of activity
25 - 35
-
2 h, residual enzyme activities are more than 90%
25 - 65
-
half-life of 81 min at 25°C, 77 min at 35°C, 64 min at 45°C, 36 min at 55°C, and 21 min at 65°C
25 - 70
stable at, inactivation above
30 - 50
2 h, stable
30 - 60
-
stability between 30°C and 60°C with a maximum at 50°C
4
-
stable up to 12 days
4 - 45
the enzyme is most stable between 4°C and room temperature. After 288 h, the recovered activities correspond to approximately 51% at 4°C, 57% at 15°C and 49% at room temperature of the initial activity. The laccase is nearly completely inactivated at 35°C after 288 h and at 45°C after 24 h
4 - 70
-
after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 100% activity at 4°C, about 90% activity at 30°C, about 75% activity at 40°C, about 50% activity at 50°C, about 5% activity at 60°C, and no activity at 70°C
40 - 50
-
most stable at a temperature range of 40-50°C
40 - 60
45 - 50
the enzyme retains 35 to 60% of its activity at 45°C to 50°C after incubation for 2.5 days
45 - 52
-
melting temperature in presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol
45 - 55
-
2 h, the enzyme retains more than 60% of its activity
45 - 70
-
the enzyme is activated by 10 min of preincubation at 45-70°C, but higher temperatures promote inactivation
50 - 70
-
the enzyme retains activity after incubation at 50°C for 1 h, but the activity sharply decreases when the temperature is increased to 60°C and almost no activity is detected at 70°C. The laccase can still oxidize 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) when incubated at 70°C for 5 min, but when the enzyme is incubated under the same condition without substrate, it loses almost all its activity
50 - 80
the Lac activity of Lac1 remains 80% after incubation at 50°C for 30 min. However, incubated at 60°C, the remaining activity declines in less than 80%. The enzyme is inactive at 70-80°C
53
-
half life 220 sec
61.7
-
T50-value, mutant enzyme V159E/N398D/I453F/M454L
62.5
-
T50-value, mutant enzyme V159E/N398D/I453L/M454L
65 - 75
-
2 h, the enzyme loses nearly 80% of its activity
68.8
-
T50-value, mutant enzyme N398D
69.9
-
T50-value, wild-type enzyme
70 - 80
-
with syringaldazine as the substrate, the activity is increased 3fold after 50 min preincubation at 70°C and 2.4fold after 10 min preincubation at 80°C. Preincubation of the enzyme in 70°C for 30 min raises the activity 4fold with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as the substrate
95
-
half-life: 2 h, recombinant enzyme
additional information