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0 - 25
-
purified recombinant enzyme, stable
0 - 50
-
purified enzyme, stable
10
-
1 h, 5% loss of activity
10 - 30
-
at pH 6, the rate of inactivation increases as the temperature is elevated from 10 to 60°C, with good stability observed in the range of 10 to 30°C
20 - 30
16 h, less than 5% loss of activity
20 - 50
-
purified enzyme, 90 min, completely stable
20 - 70
temperature stability profiles of native and deglycosylated enzymes, overview
20 - 80
stable from 20°C-80°C
22
-
50 h, about 55% loss of activity
25 - 35
-
2 h, residual enzyme activities are more than 90%
25 - 65
-
half-life of 81 min at 25°C, 77 min at 35°C, 64 min at 45°C, 36 min at 55°C, and 21 min at 65°C
25 - 70
stable at, inactivation above
30 - 60
-
stability between 30°C and 60°C with a maximum at 50°C
4 - 45
the enzyme is most stable between 4°C and room temperature. After 288 h, the recovered activities correspond to approximately 51% at 4°C, 57% at 15°C and 49% at room temperature of the initial activity. The laccase is nearly completely inactivated at 35°C after 288 h and at 45°C after 24 h
4 - 70
-
after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 100% activity at 4°C, about 90% activity at 30°C, about 75% activity at 40°C, about 50% activity at 50°C, about 5% activity at 60°C, and no activity at 70°C
40 - 50
-
most stable at a temperature range of 40-50°C
45 - 50
the enzyme retains 35 to 60% of its activity at 45°C to 50°C after incubation for 2.5 days
45 - 52
-
melting temperature in presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol
45 - 55
-
2 h, the enzyme retains more than 60% of its activity
45 - 70
-
the enzyme is activated by 10 min of preincubation at 45-70°C, but higher temperatures promote inactivation
50 - 70
-
the enzyme retains activity after incubation at 50°C for 1 h, but the activity sharply decreases when the temperature is increased to 60°C and almost no activity is detected at 70°C. The laccase can still oxidize 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) when incubated at 70°C for 5 min, but when the enzyme is incubated under the same condition without substrate, it loses almost all its activity
50 - 80
the Lac activity of Lac1 remains 80% after incubation at 50°C for 30 min. However, incubated at 60°C, the remaining activity declines in less than 80%. The enzyme is inactive at 70-80°C
61.7
-
T50-value, mutant enzyme V159E/N398D/I453F/M454L
62.5
-
T50-value, mutant enzyme V159E/N398D/I453L/M454L
65 - 75
-
2 h, the enzyme loses nearly 80% of its activity
68.8
-
T50-value, mutant enzyme N398D
69.9
-
T50-value, wild-type enzyme
70 - 80
-
with syringaldazine as the substrate, the activity is increased 3fold after 50 min preincubation at 70°C and 2.4fold after 10 min preincubation at 80°C. Preincubation of the enzyme in 70°C for 30 min raises the activity 4fold with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as the substrate
95
-
half-life: 2 h, recombinant enzyme
100
-
1 h, 80% loss of activity
100
-
purified native enzyme, 10 min, inactivation
100
inactivation through protein unfolding. The enzyme is refolded at 25°C but rests catalytically inactive
100
-
half-life is 8.7 min
100
MK290990.1
after 90 min enzyme activity increases to 138.76% and subsequently decreases, displaying residual activity of 69.90% at 270 min
20
-
purified laccase is stable at 20°C, pH 7.0 and pH 3.0
20
-
1 h, 4% loss of activity
25
-
purified native enzyme, half-life is 116 min
25
-
purified isozymes Lacc I and Lacc II are stable
25
-
150h, 50% residaul activity
30
-
60 min, enzyme retains 100% of its activity
30
24 h, 10% loss of activity
30
-
1 h, 8% loss of activity
30
-
40 h, 70% loss of activity
30
-
24h, 100% residual activity, increase in activity by 80% after shift from room temperature to 30°C
30
-
pH 6.0, stable for 16 h
30
-
purified enzyme, highly stable at
30
-
stable over a broad range of temperatures and are most stable at 30°C
30
Sporothrix carnis
-
the enzyme retains 94% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
30
-
8 h, 40% loss of activity
30
-
the enzyme shows 1.3fold thermal activation within 360 min of incubation
35
-
purified native enzyme, half-life is 104 min
35
-
1 h, about 20% loss of activity
35
-
purified recombinant enzyme, 2 h, loss of 30% activity
35
-
1 h, about 50% loss of activity
35
-
purified enzyme, 24 h, stable
37
2.5 d, the enzyme retains nearly all of its original activity
37
30 days, the enzyme retains 80% of its activity
40
-
purified laccase rapidly loses its activity at 40°C
40
half-life time (t1/s): more than 12 h
40
-
60 min, enzyme retains 97% of its activity
40
-
1 h, 1% loss of activity
40
-
20h, 50% residual activity
40
-
80 h, about 50% loss of activity
40
-
24h, 100% residual activity, increase in activity by 100% after shift from room temperature to 40°C
40
-
stable for at least 12 h
40
half-life of recombinant, expressed in Saccharomyces cerevisiae, wild-type enzyme is over 50 h, and of the L559A mutant is 24 h
40
-
pH 3, 1 h, about 15% loss of activity
40
Phlebia fascicularia
-
stable below
40
-
POXA3a, 50% residual activity after 6 h, POXA3b, 50% residual activity after 14h
40
-
1 h, 50% loss of activity
40
-
3 h, at temperatures below 40°C, there is almost no change in enzymatic activity
40
Sporothrix carnis
-
the enzyme retains 98% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
40
-
30 min, 52% loss of activity
40
-
8 h, 60% loss of activity
40
Thermochaetoides thermophila
-
half-life: 24 h
40
-
the enzyme shows 1.3fold thermal activation within 90 min of incubation
40
16 h, about 65% loss of activity
40
-
30 min, about 20% loss of activity
40
-
purified native enzyme, 60 min, completely stable up to
40
-
purified enzyme, stable up to
40 - 60
recombinant enzyme, pH 5.0 for 9 h, stable
40 - 60
-
isoform POXA1b shows a inactivation half-period of 3 h at 60°C, isoform POXA1w shows a inactivation half-period of 3.3 h at 60°C, isoform POXA2 shows a inactivation half-period of 0.2 h at 60°C, isoform POXA3a shows a inactivation half-period of 6 h at 40°C, isoform POXA3b shows a inactivation half-period of 14 h at 40°C, isoform POXC shows a inactivation half-period of 30 min at 40°C
45
-
purified native enzyme, half-life is 67 min
45
-
2 h, 20% loss of actuvity
45
-
purified recombinant enzyme, 1 h, loss of 30% activity
45
-
the enzyme is most stable at 45°C when exposed for 1 h
50
-
half-life 3 h
50
-
60 min, more than 80% residual activity
50
-
30 min, enzyme retains 85.4% of its activity. 90 min, enzyme retains 80% of its activity
50
6 h, 50% loss of activity
50
-
a significant increase in activity is observed until 60 min, in which the relative activity reaches to 150%
50
-
1 h, no loss of activity
50
10 h, no loss of activity
50
t1/2: 40.3 h (wild-type PM1 laccase), t1/2: 21.7 h (mutant 7D5 laccase)
50
-
1 h, 25% loss of activity
50
-
laccase Lac-4.8 shows thermal activation at 50°C and the enzyme activity after incubation is up to 120% of the original activity measured at 25°C
50
150 min, 85% of the original enzyme activity is retained
50
Tm of Lcc3 is determined at 73.9°C and it shows a t1/2 of 120 min at 50°C
50
-
60 min, purified isozyme Lacc I shows loss of 20% activity, purified isozyme Lacc II is stable
50
recombinant laccase is stable at temperatures below 50°C
50
30 min, no loss of activity, both wild-type and region Pro357-His406 deletion mutant
50
-
20 h, 80% loss of activity
50
fully active after 5 h at 50°C. Half-life: 1 day
50
-
purified enzyme, unstable above
50
-
purified enzyme, half-life: 281 min
50
half-life of recombinant, expressed in Saccharomyces cerevisiae, wild-type enzyme is 23 h, and of the L559A mutant is 6 h
50
-
1 h, about 65% loss of activity
50
-
pH 3, 1 h, about 80% loss of activity
50
-
inactivated rapidly above
50
the enzyme retains 100% activity after 3 h
50
-
60 min, 70% residual activity
50
-
210 min, 50% residual activity
50
-
3 h, at temperatures over 50°C the remaining laccase activity drops to below 70% of the initial activity
50
-
10 min, about 80% loss of activity
50
-
30 min, 75% loss of activity
50
-
8 h, 70% loss of activity
50
Thermochaetoides thermophila
-
half-life: 12 h
50
-
purified free enzyme half-life: 140 min, purified enzyme conjugated with 1.1 kDa PEG half-life: 70 min, purified enzyme conjugated with 2 kDa PEG half-life: 150 min, purified enzyme conjugated with 5.0 kDa PEG half-life: 210 min
50
-
20 min, 25% loss of activity
50
-
purified native enzyme, 60 min, 10% loss of activity
50
-
purified enzyme, 93% remaining activity after 24 h, 76% remaining activity after 48 h
50
-
purified enzyme, rapid decrease in activity
50
after 20 min the enzyme loses 60% of its initial activity
50
-
4 h, soluble and immobilized laccases retain more than 90% activity at 50°C. Copper alginate shows better stability than calcium alginate immobilized laccase
55
-
purified native enzyme, half-life is 47 min
55
-
loss of 80% activity, half-life: 12 min
55
the enzyme is fully active after 1 h at 55°C
55
-
incubation of enzyme above 55 °C initiates denaturing of enzyme and conformational changes, leveraging the decreased enzyme activity
55
Thermochaetoides thermophila
-
stable below
55
-
loss of 50% activity after 90 min
55
-
4 h, calcium alginate-chitosan immobilized laccase retains 91% activity
55
4 h, the relative enzyme activity is still over 50%, mutant enzyme V281A/P309L/S318G/D232V
60
-
half-life 40 min
60
-
10 h, almost complete inactivation
60
-
60 min, 25% residual activity
60
-
30 min, enzyme retains 54.0% of its activity. 90 min, enzyme retains 48% of its activity
60
-
pH 4.5, 60 min, 50% residual activity
60
stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
60
360 min, 40% loss of activity
60
-
after 15 min incubation relative activity reaches to 140%
60
-
1 h, 9% loss of activity
60
10 h, no loss of activity
60
t1/2: 4.85 h (wild-type PM1 laccase), t1/2: 2.8 h (mutant 7D5 laccase)
60
-
1 h, 18% loss of activity
60
-
Lac-3.5 and Lac-4.8 show thermal stability at up to 60°C
60
150 min, 56% of the original enzyme activity is retained
60
recombinant enzyme, stable up to
60
the stability of laccase is not affected by temperatures below 60°C
60
pH 4.5, half-life of wild-type enzyme Lcc9 is 24 min, half-life of the recombinant enzyme expressed in Pichia pastoris is 15 min
60
the enzyme retains 100% of its initial activity after 3 h of incubation
60
-
complete inactivation, half-life: 4 min
60
30 min, 50% loss of activity, both wild-type and region Pro357-His406 deletion mutant
60
1 h, 10% loss of activity
60
-
purified enzyme, 90 min, 65% remaining activity
60
-
30 min, 33% loss of activity
60
-
purified enzyme, half-life: 25 min
60
Loweporus lividus
-
the enzyme is stable at 60°C for 2 h
60
Loweporus lividus MTCC-1178
-
2 h, stable
60
half-life of recombinant, expressed in Saccharomyces cerevisiae, wild-type enzyme is 4.5 h, and of the L559A mutant is below 10 min
60
-
10 min, about 85% loss of activity
60
-
5 h, 50% residual activity
60
-
pH 3, 1 h, complete loss of activity
60
-
60 min, 15% residual activity
60
-
POXA3a and POXA3b, 50% residual activity after a few min
60
-
purified enzyme, 10 min, loss of 90% activity
60
-
23 min, 50% residual activity
60
-
1 h, complete loss of activity
60
-
10 min, about 85% loss of activity. 40 min, complete inactivation
60
Sporothrix carnis
-
the enzyme retains 91% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
60
-
purified isozymes, half-lives are 5-6 h, after 8 h 20% remaining activity for isozyme laccase I, 40% for laccase II and III
60
1 h, 50% loss of activity
60
-
8 h, 90% loss of activity
60
-
6 h, the enzyme retains 50% of its initial activity
60
-
20 min, about 80% loss of activity
60
-
purified enzyme, 2 h, 80% remaining activity
60
-
stable below, temperature stability profile
60
-
14 min, 50% residual activity
60
-
1 h, loss of 40% activity, purified enzyme
60
-
pH 5.0, half-life is 74 min
60
1 h, residual activity is more that 50%
65
-
purified native enzyme, half-life is 33 min
65
-
24 h, completely stable
65
the enzyme undergoes slow partial unfolding and thermal inactivation above 65°C, making it unsuitable for long incubations above this temperature
65
-
loss of 50% activity after 30 min
65
-
purified enzyme, half-life is 60 min
65
-
4 h, sol-gel immobilized laccase retains 90% activity
65.8
-
T50-value, mutant enzyme V159E
65.8
-
T50-value, mutant enzyme V159E/N398D
70
-
half-life 10 min
70
the mutant enzyme is resistant to heat treatment of 10 min. The mutant protein loses 45% of its original activity after incubation for 1 h. Mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence
70
half-life time (t1/s): 30 min
70
-
30 min, enzyme retains 15.0% of its activity. 90 min, enzyme retains 10% of its activity. 180 min, complete inactivation
70
-
1 h, 57% loss of activity
70
wild-type and mutant enzymes retain more than 50% activity on both 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) and guaiacol upon incubation at 70 or 80°C for 2h
70
-
after 15 min incubation relative activity reaches to 148%
70
half-life: 60 min, recombinant enzyme
70
-
1 h, 21% loss of activity
70
-
after incubation for 10 h the residual activity is greater than 50%. Activity increases by more than 120% after 1 h incubation
70
10 h, more than 50% of activity
70
t1/2: 1.6 h (wild-type PM1 laccase), t1/2: 0.22 h (mutant 7D5 laccase)
70
-
1 h, 47% loss of activity
70
-
half-life: 30 min, laccase Lac-4.8
70
-
half-life: 60 min, laccase Lac-3.5
70
inactivated after 20 min
70
-
20 min, purified isozyme Lacc I shows complete loss of activity, isozyme Lacc II 10% after 60 min
70
recombinant enzyme, pH 5.0 for 9 h, loss of 80% activity
70
1 h, 40% loss of activity
70
-
purified enzyme, half-life: 4 min
70
-
100 mim, 20% loss of activity
70
FJ560721
the purified native enzyme retains 50% of its activity after 120 min of incubation
70
-
15 min, 50% residual activity
70
-
2 h, complete inactivation
70
-
purified enzyme, 10 min, 70% activity remaining
70
-
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4
70
Sporothrix carnis
-
the enzyme retains 84% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
70
-
purified isozymes, 8-15% remainng activity after 4 h, 50% remaining activity after 1.7 h
70
-
30 min, complete inactivation
70
-
purified recombinant enzyme, 90% remaining activity after 25 min, 50% remaining actiivty after 110 min
70
-
8 h, complete loss of activity
70
-
60 min, 40% residual activity
70
Thermochaetoides thermophila
-
1 h, stable
70
-
5 min, 90% loss of activity
70
-
purified native enzyme, 60 min, inactivation
70
-
purified enzyme, 2 h, 40% remaining activity
75
-
purified native enzyme, half-life is 23 min
75
mutant 7D5 laccase is notably activated at high temperature (laccase activity increases around 60% during the first 5 min of incubation at 75°C) whereas PM1 laccase is not
75
-
2 h, 97% loss of activity
75
-
40 min, enzyme hardly retains any activity
75
-
purified recombinant enzyme, 5 min, loss of 90% activity
75
-
purified enzyme, inactivation
75
27 min, 50% residual activity
79
-
melting temperature at pH 7.0. Presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol reduces melting temperature to 45-52°C
79
T50: 10 min, wild-type PM1 laccase
80
10 min, mutant enzyme retains 70% of its original activity, mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence
80
10 min, complete inactivation
80
-
90 min, complete inactivation
80
-
pH 4.5, less than 2 min, 50% residual activity
80
-
1 h, 92% loss of activity
80
90 min, 25% loss of activity
80
wild-type and mutant enzymes retain more than 50% activity on both 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) and guaiacol upon incubation at 70 or 80°C for 2h
80
half-life: 20 min, recombinant enzyme
80
-
1 h, 44% loss of activity
80
half-life: 2.5 h, 21 % of the initial activity is detected after 10 h
80
-
ativity increases by nearly 90% after 1 h incubation
80
t1/2: 0.12 h (wild-type PM1 laccase), t1/2: 0.03 h (mutant 7D5 laccase)
80
-
1 h, complete loss of activity
80
-
20 min, 80% loss of activity
80
inactivated after 2 min
80
-
rapid inactivation of purified isozyme Lacc II with 95% loss of activity within 10 min
80
the remaining activity is 37% and 7% following treatment for 10 min and 60 min
80
Sporothrix carnis
-
the enzyme retains 65% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
80
30 min, wild-type enzyme shows 60% of initial activity after incubation, mutant enzymes M199G and Y230A exhibit less than 30% of the initial activity
80
Thermochaetoides thermophila
-
half-life: 10 min
80
half-life: more than 14 h
80
-
0-4 h, residual enzymatic activity decreases with the increase of the incubation time. The wild-type, K428M, K428E and K428R enzymes remain 70% of corresponding initial activity after incubated for 4 h. The K428L mutant is most affected, only 30% of the initial activity is retained after 4 h
80
4 h, wild-type enzymed retains 80% of its activity. The thermal stability of D394R mutant decreases significantly, with the residual activity of 15% after 4 h
80
-
purified enzyme, 2 h, 5% remaining activity, half-life at 80°C is 20 min
85
10 min, mutant enzyme retains 30% of its original activity, mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence
85
-
10 min, complete inactivation
85
-
purified enzyme, 10 min, inactivation
90
35 min, 30% loss of activity
90
-
1 h, 68% loss of activity
90
-
2 h, recombinant enzyme retains 85% of its activity
90
above, high thermal stability of purified wild-type rMmPPOA-695-His and mutant rMmPPOA-637-His enzymes
90
Sporothrix carnis
-
the enzyme retains 50% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
additional information
-
T1 copper depletion is a key event in inactivation and a determinant of the thermodynamic stability, thermodynamic stability of wild-type and mutant proteins, overview
additional information
-
laccase 2 has higher thermostability than laccase 1
additional information
the recombinant His-tagged MmPPOA-695 shows a very high thermal stabilit
additional information
-
the recombinant His-tagged MmPPOA-695 shows a very high thermal stabilit
additional information
thermal stability of the enzyme is negatively influenced by an alteration of the amino acid sequence at the C terminus. Glycosylation enhances the thermal stability of the enzyme
additional information
-
thermal stability of the enzyme is negatively influenced by an alteration of the amino acid sequence at the C terminus. Glycosylation enhances the thermal stability of the enzyme
additional information
-
the activation energy for thermal denaturation of the enzyme is 38.20 kJ/mole/K