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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Cloned

Cloned on EC 1.10.3.2 - laccase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
attempts of heterologous expression of the wild-type laccase using a Pichia pastoris secretory expression system are unsuccessful most likely because the enzyme is too unstable and degrades immediately after production. Therefore the stability of the laccase is improved by using a phylogeny-based design method. A mutant laccase is created in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence. The resulting mutant protein is successfully produced using the Pichia pastoris secretory expression system and then purified
by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23. The DNA encoding the mature protein is then cloned and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme, expressed as an apoprotein, is dialyzed against copper-containing buffer to yield a holoprotein
cloned and expressed in Pichia pastoris
CotA-type laccase, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, primary structure of the gene, sequence comparisons
-
DNA and amino acid sequence determination and analysis, sequence comparison
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain Top10, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain W303-1A
efficiently expressed in Escherichia coli BL21(DE3) and process is developed to harvest maximum potential of the enzyme by the refolding of the inclusion bodies
efficiently expressed in Escherichia coli in a biologically active form
enzyme expression in Trichoderma reesei under the strong cbh1 (cel7A) promoter in a strain in which the major cellulase gene cbh1 is disrupted
-
enzyme is synthesized as precursor
-
Ery4 genetic structure, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae
Escherichia coli expression system BL21(DE3)
expressed in Escherichia coli
-
expressed in Escherichia coli DH5alpha cells
expressed in Saccharomyces cerevisiae using an improved signal peptide previously obtained and enzyme directed evolution
-
expression highly induced by copper
-
expression in Aspergillus sp.
-
expression in Escherichia coli
expression in Escherichia coli as soluble intein fusion protein
-
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli BL21 DE3
-
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli or in Pichia pastoris
expression in in Pichia pastoris by fusing an additional ten amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser) tag at N-terminus
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae EBY100
-
expression in Trichoderma reesei
expression of a His-tagged two-type domain laccase, lacking the signal sequence domain, in Escherichia coli strain BL21(DE3)
-
expression of a soluble and functional laccase in modified Escherichia coli, trxB2/gor2 mutant(OrigamiTMB (DE3)) at the expression level suitable for industrial application. The choice of Origami is dictated by the fact that disulfide bridges formation is necessary for properfolding of laccase
expression of full-length and amino-terminally truncated laccase-2, both showing lower activity than the native enzyme
expression of laccase 3 variants in Saccharomyces cerevisiae
expression of laccase Lcc4/1 (composed of the N-terminus of the Lcc4 and the C-terminus of the Lcc1 laccases) in tobacco cell culture
expression of lcc3 gene in Komagataella (i.e. Pichia) pastoris
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) resulting in strains AH3547 and AH3560
-
expression of wild-type and mutant enzymes in Trichoderma reesei and Saccharomyces cerevisiae, expression of mutant L559A in Saccharomyces cerevisiae. The yeast is not able to process MaL correctly, and the additional 14 amino acids are present in the protein. Therefore, expression is performed with another construct, pMS175, where mature MaL cDNA, with a stop codon, is introduced after the C-terminal processing site. Changes in the C-terminus of MaL cause major defects in protein production in both expression hosts
expression of wild-type and mutant enzymes in Yarrowia lipolytica Po1t prototrophic strain
four distinct genes
-
functional enzyme expression in Saccharomyces cerevisiae, expression of engineered mutant enzymes in Aspergillus niger, the evolved variants are highly
gene CcLCC5I, expresssion in Pichia pastoris GS115
gene lac1, DNA and amino acid sequence determination and analysis, genomic organization, and expression analysis in host roots, overview
gene lac1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris and Escherichia coli strain DH5aalpha
gene lac1, genomic organization, and expression analysis in host roots, overview
gene lac2, DNA and amino acid sequence determination and analysis
gene lac2, DNA and amino acid sequence determination and analysis, genomic organization, and expression analysis in host roots, overview
gene lac2, genomic organization, and expression analysis in host roots, overview
gene lac3, DNA and amino acid sequence determination and analysis, genomic organization, and expression analysis in host roots, overview
gene lac3, genomic organization, and expression analysis in host roots, overview
gene lac5, DNA and amino acid sequence determination and analysis, genomic organization, and expression analysis in host roots, overview
gene lac5, genomic organization, and expression analysis in host roots, overview
gene lcc1 or lccalpha, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of a soluble enzyme in Saccharomyces cerevisiae strain W303-1a
gene lcc1, DNA and amino acid sequence determination and analysis, tissue-specific expression analysis by realtime PCR, functional ectopic expression of both wild-type and chimeric Lcc1 in Nicotiana tabacum BY-2 cells using the CaMV35S promoter and secretion of the recombinant enzymes
gene lcc1, expression in Aspergillus oryzae as precursor enzyme
-
gene lcc3, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene ppoA, the full-length and truncated enzyme is overexpressed in Escherichia coli strain Rosetta 2(DE3) and BL21(DE3) with and without a C-terminal His6-tag, method optimization, overview
genes lac1 and lac2, DNA and amino acid sequence determination and analysis, expression of Lac1 in Pichia pastoris
heterogeneously expressed in insect Sf9 cells
heterologous expression in Aspergillus niger
heterologous expression of lcc9 in Pichia pastoris GS115
heterologously expressed in Escherichia coli
heterologously overexpressed in Escherichia coli
-
heterologously produced using Trichoderma reesei QM9414 DELTAxyr1 as expression host
His6-tagged enzyme is heterologously expressed in Escherichia coli
lac90, lac110 and three additional cDNAs
-
Laccase-encoding cDNA, expression and procedure optimization of a recombinant His-tagged laccase expressed in supercompetent Escherichia coli strain B F-dcm ompT hsdS (rB-, mB-) gal lambda (DE3)
-
lacG is cloned and expressed as a fusion protein with six histidine residues in Escherichia coli BL21 (DE3) cells
-
overexpression in Escherichia coli
overexpression in Pichia pastoris
overexpression of a truncated enzyme mutant, lacking the first 31 amino acids, in Pichia pastoris strain GS115
-
overproduced in Escherichia coli
recombinant expression in Streptomyces lividans, the enzyme is secreted
-
the expression plasmids (pJAM822 and pJAM824) are transformed into Haloferax volcanii H26 to generate strains SB01 and US02 for high-level synthesis of LccA with and without a C-terminal StrepII tag
the lccA gene sequence is mutated to encode LccA with either a modified twin-arginine translocation (TAT) motif (R6K R7K R8K or Dtat) or deletion of its N-terminal propeptide (DMet1 to Ala31 of the deduced polypeptide or Dpro). With this approach, the enzyme is produced at high levels in recombinant Escherichia coli grown in medium supplemented with 0.25 mM CuSO4
transformed into Escherochia coli BL21(DE3)