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0.00018
-
substrate caffeic acid or catechol, leaf enzyme extract
0.00054
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.00084
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.0009
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.0012
-
substrate catechol, leaf enzyme extract
0.0022
-
substrate catechol, leaf enzyme extract
0.0045
-
substrate catechol, leaf enzyme extract
0.0063
-
substrate catechol, leaf enzyme extract
0.0065
-
substrate catechol, leaf enzyme extract
0.007
-
substrate 5-caffeoyl quinic acid or 4-methylcatechol, leaf enzyme extract
0.01
-
substrate catechol, leaf enzyme extract
0.011
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.0124
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.014
Coffea guarini
-
substrate caffeic acid, leaf enzyme extract
0.016
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.017
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.018
-
substrate 4-methylcatechol, leaf enzyme extract
0.019
-
substrate 4-methylcatechol, leaf enzyme extract
0.02
-
substrate 4-methylcatechol, leaf enzyme extract
0.026
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.03
-
substrate 4-methylcatechol, leaf enzyme extract
0.036
-
substrate catechol, leaf enzyme extract
0.04
-
substrate 4-methylcatechol, leaf enzyme extract
0.045
Coffea guarini
-
substrate catechol, leaf enzyme extract
0.073
Coffea guarini
-
substrate 4-methylcatechol, leaf enzyme extract
0.09
Coffea guarini
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.145
-
using L-DOPA as substrate, crude enzyme, at pH 7.5 and 25°C
1
-
reaction with catechol
1.32
-
crude extract, at pH 7.0, 25°C
10
Mycelia sterilia
-
reaction with (-)-epigallocatechin
13.15
Ferula sp.
-
purified stem enzyme
18.18
-
purified recombinant enzyme
18.7
-
partially purified soluble, active enzyme form, substrate tert-butyl-catechol
2.2
Mycelia sterilia
-
reaction with catechol
204.69
-
purified enzyme, substrate chlorogenic acid, pH 5.0, 20°C
227700
-
highly purified enzyme, substrate catechol
24.1
-
solubilized, crude enzyme
241.5
-
isozyme PPO 2 after 183fold purification, at pH 7.0, 25°C
4.458
-
using L-DOPA as substrate, after 30.76fold purification, at pH 7.5 and 25°C
4.5
-
reaction with pyrogallol
41.6
Ferula sp.
-
purified leaf enzyme
5.5
-
reaction with (-)-epigallocatechin gallate
5.7
Mycelia sterilia
-
reaction with pyrogallol
7.3
-
reaction with (-)-epigallocatechin
788.3
-
partially purified enzyme
8.7
Mycelia sterilia
-
reaction with (-)-epigallocatechin gallate
8.76
-
isozyme PPO 1 after 6.6fold purification, at pH 7.0, 25°C
9.8
-
pH 6.0, 40°C, enzyme extract
91.5
-
purified particulate, latent enzyme form, substrate tert-butyl-catechol
0.00042
-
substrate caffeic acid, leaf enzyme extract
0.00042
-
substrate coumaric acid, leaf enzyme extract
0.0006
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.0006
Coffea guarini
-
substrate coumaric acid, leaf enzyme extract
0.0006
-
substrate coumaric acid, leaf enzyme extract
0.0014
-
substrate caffeic acid, leaf enzyme extract
0.0014
-
substrate caffeic acid, leaf enzyme extract
0.002
-
substrate caffeic acid, leaf enzyme extract
0.002
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.002
-
substrate 4-methylcatechol, leaf enzyme extract
0.002
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.003
-
substrate 4-methylcatechol, leaf enzyme extract
0.003
Coffea guarini
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.004
-
substrate caffeic acid, leaf enzyme extract
0.004
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.004
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.004
-
substrate caffeic acid, leaf enzyme extract
0.005
-
substrate caffeic acid, leaf enzyme extract
0.005
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.006
-
substrate catechol, leaf enzyme extract
0.006
-
substrate caffeic acid, leaf enzyme extract
0.006
-
substrate caffeic acid, leaf enzyme extract
0.008
-
substrate catechol, leaf enzyme extract
0.008
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.008
-
substrate caffeic acid, leaf enzyme extract
0.0083
-
substrate 4-methylcatechol, leaf enzyme extract
0.0083
-
substrate 4-methylcatechol, leaf enzyme extract
0.023
-
substrate 4-methylcatechol, leaf enzyme extract
0.023
-
substrate catechol, leaf enzyme extract
0.051
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
0.051
-
substrate 5-caffeoyl quinic acid, leaf enzyme extract
additional information
-
-
additional information
-
-
additional information
-
46000 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.001 per min
additional information
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the specific activity of the crude enzyme is 3.72 units/mg, the specific activity of the 28.24fold purified enzyme is 105 units/mg, one unit is defined as an increase in the rate of absorbance of 0.001 per min, using L-dopa as substrate, at pH 7.1 and 28°C
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
Coffea guarini
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
assay optimization
additional information
-
-
additional information
Ferula sp.
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substrate specificity, tissue specific activities, overview
additional information
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compared to PPO from R405-2000, a nonembryogenic cultivar, PPO from coker 312 (embryogenic cultivar) shows a higher PPO activity increasing markedly form primary culture to third subculture
additional information
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68077.0 units/mg, 1 unit is defined as absorbance change of 0.001 per min at 25°C
additional information
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370004 U/mg for the purified native enzyme. One unit of enzyme activity is defined as a 0.001-unit change in absorbance per min per ml
additional information
-
-
additional information
-
-
additional information
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13160.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min
additional information
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10512.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min and per ml enzyme solution
additional information
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-
additional information
-
-
additional information
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4500 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.01 per min
additional information
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isoenzyme Ia, 123 units/mg, isoenzyme Ib, 113 units/mg, isoenzyme II, 242 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min
additional information
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isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min
additional information
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analysis of browning parameters
additional information
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-
additional information
-
-
additional information
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polyphenol oxidases are induced in cowpea plants by wounding. The highest activity levels are detected 48 h after this stimulus in both wounded and neighbor-to-wounded unifoliates of cowpea seedlings. The increase of activity is in the order of 13 to 15fold, respectively, in comparison to control unifoliates
additional information
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2612.06 U/mg, one enzyme unit is defined as the amount of enzyme that produces a rise of 0.001 absorbance in one minute at 420 nm