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36.5
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
115 - 150
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
148.3 - 233.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
78.3 - 216.7
cellopentaose
56.7 - 233.3
cellotetraose
0.008 - 0.2
N-acetyl-D-glucosamine
73.3 - 233.3
xylotetraose
115
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
150
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
148.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
233.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
30
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
56.7
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
56.7
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
68.3
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
100
cellobiose
wild type enzyme, at 37°C and pH 8.0
116.7
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
156.7
cellobiose
wild type enzyme, at pH 8.0 and 37°C
306.7
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
66.7
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
91.7
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
150
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
78.3
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
145
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
216.7
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
56.7
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
86.7
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
150
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
233.3
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.25
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
23.3
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
45
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
45
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
48.3
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
70
cellotriose
wild type enzyme, at 37°C and pH 8.0
93.3
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
143.3
cellotriose
wild type enzyme, at pH 8.0 and 37°C
250
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
D-galactose
wild type enzyme, at 37°C and pH 8.0
0.11
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
0.14
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
0.18
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
0.18
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
0.4
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.42
D-glucose
wild type enzyme, at pH 8.0 and 37°C
0.43
D-glucose
wild type enzyme, at 37°C and pH 8.0
0.83
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
1.6
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
1.63
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
2.883
D-glucose
pH 8.0, 37°C, recombinant enzyme mutant
3.6
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
8.1
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
0.02
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
0.05
D-xylose
wild type enzyme, at 37°C and pH 8.0
0.05
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.06
D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.11
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.22
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
0.22
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.36
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
0.45
D-xylose
pH 8.0, 37°C, recombinant enzyme mutant
51.8
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
0.53
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
0.95
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
1.1
maltose
mutant enzyme W351F, at 37°C and pH 8.0
2.13
maltose
wild type enzyme, at 37°C and pH 8.0
0.008
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
0.02
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
0.14
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
0.2
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
2.48
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
2.6
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
2.6
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
5.11
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
23.3
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
75
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
90
xylobiose
wild type enzyme, at 37°C and pH 8.0
151.7
xylobiose
wild type enzyme, at pH 8.0 and 37°C
300
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
73.3
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
135
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
300
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
85
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
196.7
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
283.3
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
73.3
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
136.7
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
233.3
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
2.83
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
3.15
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
4.3
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
4.33
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
25
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
85
xylotriose
wild type enzyme, at 37°C and pH 8.0
85
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
136.7
xylotriose
wild type enzyme, at pH 8.0 and 37°C
183.3
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C