1.1.99.31: (S)-mandelate dehydrogenase
This is an abbreviated version!
For detailed information about (S)-mandelate dehydrogenase, go to the full flat file.
Word Map on EC 1.1.99.31
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1.1.99.31
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s-mandelate
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putida
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benzoylformate
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fmn-dependent
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benzaldehyde
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calcoaceticus
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fmn
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mitra
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alpha-hydroxy
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l-lactate
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phenylglyoxylate
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carbanion
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flavocytochrome
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membrane-binding
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flavoenzyme
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alpha-protons
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half-reaction
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rhodotorula
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graminis
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electron-rich
- 1.1.99.31
- s-mandelate
- putida
- benzoylformate
-
fmn-dependent
- benzaldehyde
- calcoaceticus
- fmn
- mitra
-
alpha-hydroxy
- l-lactate
- phenylglyoxylate
-
carbanion
-
flavocytochrome
-
membrane-binding
-
flavoenzyme
-
alpha-protons
-
half-reaction
-
rhodotorula
- graminis
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electron-rich
Reaction
Synonyms
(S)-mandelate dehydrogenase, L-mandelate dehydrogenase, L-MDH, MDH, SManDH, SMDH
ECTree
1.1.99.31 (S)-mandelate dehydrogenaseAdvanced search results
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results (Turnover Number
Turnover Number on EC 1.1.99.31 - (S)-mandelate dehydrogenase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.03
(R,S)-p-chloromandelate
-
mutant enzyme H274G, in presence of 20 mM imidazole
0.5
2-Hydroxy-3-butenoate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.03
2-Hydroxyisovalerate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.23
(S)-Mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
1.6
(S)-Mandelate
-
pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach
2.3
(S)-Mandelate
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pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH
3 - 6
(S)-Mandelate
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pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol
9
(S)-Mandelate
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pH 7.5, (S)-mandelate dehydrogenase with residues 2-4 deleted
24
(S)-Mandelate
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pH 7.5, (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus
66
(S)-Mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
205
(S)-Mandelate
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pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
205
(S)-Mandelate
-
pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
290
(S)-Mandelate
-
pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme
360
(S)-Mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
6.6
2-Hydroxy-3-butynoate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.1
2-Hydroxybutyrate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.48
2-hydroxyhexanoate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
1.28
2-hydroxyisocaproate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.96
2-Hydroxyoctanoate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.5
2-hydroxyvalerate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
3.13
3-indolelactate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
3.85
3-indolelactate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.87
3-Phenyllactate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
155
DL-[2-1H]-mandelate
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pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme
300
DL-[2-1H]-mandelate
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pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme
316
DL-[2-1H]-mandelate
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pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain
88
DL-[2-2H]-mandelate
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pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme
92
DL-[2-2H]-mandelate
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pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme
99
DL-[2-2H]-mandelate
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pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain
0.3
ethyl-(S)-mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
2.6
ethyl-(S)-mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
204
ethyl-(S)-mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
38.5
indoleglycolate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
195
Mandelate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
2.3
methyl-(S)-mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
2.5
methyl-(S)-mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
151
methyl-(S)-mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
0.03
O2
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pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.22
O2
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pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH
