1.1.99.20: alkan-1-ol dehydrogenase (acceptor)

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For detailed information about alkan-1-ol dehydrogenase (acceptor), go to the full flat file.

Reaction

Synonyms

alkan-1-ol dehydrogenase, alkan-1-ol:acceptor oxidoreductase, dehydrogenase, polyethylene glycol, ethoxy chain nonylphenol dehydrogenase, NPEO-DH, PEG-DH, PEGDH, polyethylene glycol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.20 alkan-1-ol dehydrogenase (acceptor)

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Results	in table
11	Activating Compound
3	AA Sequence
4	Application
1	CAS Registry Number
4	Cloned(Commentary)
27	Cofactor
46	Inhibitors
6	kcat/KM [mM/s]
65	KM Value [mM]
6	Localization
7	Metals/Ions
9	Molecular Weight [Da]
24	Organism
1	Pathway
4	pH Optimum
3	pH Range
7	pH Stability
5	Protein Variants
5	Purification (Commentary)
1	Reaction
3	Reaction Type
21	Reference
20	Specific Activity [micromol/min/mg]
179	Substrates and Products (Substrate)
6	Subunits
21	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Range [°C]
6	Temperature Stability [°C]
6	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 1.1.99.20 - alkan-1-ol dehydrogenase (acceptor)

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.24 - 3.6

PEG-1000

3 entries

0.54 - 5.2

PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units

3 entries

-

0.24

PEG-1000

Ensifer sp. AS08

Q2HXX0

mutant enzyme H465A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

1.9

PEG-1000

Ensifer sp. AS08

Q2HXX0

mutant enzyme N90A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

3.6

PEG-1000

Ensifer sp. AS08

Q2HXX0

wild type enzyme, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

0.54

PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units

Ensifer sp. AS08

Q2HXX0

mutant enzyme H465A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

-

1.3

PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units

Ensifer sp. AS08

Q2HXX0

mutant enzyme N90A, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

-

5.2

PEG-mono-4-nonylphenyl ether, averaging 10 ethoxy units

Ensifer sp. AS08

Q2HXX0

wild type enzyme, in 0.1 M Tris-HCl buffer (pH 8.0), at 25°C

725161

-