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1.1.98.2: glucose-6-phosphate dehydrogenase (coenzyme-F420)

This is an abbreviated version!
For detailed information about glucose-6-phosphate dehydrogenase (coenzyme-F420), go to the full flat file.

Word Map on EC 1.1.98.2

Reaction

D-glucose 6-phosphate
+
oxidized coenzyme F420
=
6-phospho-D-glucono-1,5-lactone
+
reduced coenzyme F420

Synonyms

Rv0407, coenzyme F420-dependent glucose-6-phosphate dehydrogenase, EC 1.1.99.34, F420-dependent glucose-6-phosphate dehydrogenase, FGD, FGD1, glucose-6-phosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.98 With other, known, physiological acceptors
                1.1.98.2 glucose-6-phosphate dehydrogenase (coenzyme-F420)

Purification

Purification on EC 1.1.98.2 - glucose-6-phosphate dehydrogenase (coenzyme-F420)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, F420–ether-linked aminohexyl–Sepharose 4B affinity chromatography, and quaternary aminoethyl-Sephadex column chromatography
-
HisTrap column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography and Sephacryl 200 gel filtration
recombinant His-tagged wild-type and selenomethionine-labeled FGD1 from Mycobacterium smegmatis strain mc24517 by nickel affinity chromatography and gel filtration
-