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1.1.3.7: aryl-alcohol oxidase

This is an abbreviated version!
For detailed information about aryl-alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.7

Reaction

an aromatic primary alcohol
+
O2
=
an aromatic aldehyde
+
H2O2

Synonyms

AAO, AAO2, AAOx, alcohol: O2 oxidoreductase, AOX, arom. alcohol oxidase, aryl alcohol oxidase, arylalcohol oxidase, CpSAO, CtSAO, GaoB, GLRG_02805, GMC oxidoreductase-like protein, HMFO, More, MtGloA, MYCTH_2299749, oxidase, aryl alcohol, salicyl alcohol oxidase, um04044, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.7 aryl-alcohol oxidase

Temperature Stability

Temperature Stability on EC 1.1.3.7 - aryl-alcohol oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
initial loss of 10% of activity, thereafter stable or at least 22 h
30
-
highly decreased stability beyond 30°C
30 - 70
the enzyme is stable up to 30°C and the stability is greatly reduced at 40°C (50% inactivation) and leads to 100% inactivation after 5 min at 70°C
37 - 50
the enzyme loses 86% of its activity after 12 min at 50°C, while it remains stable at 37°C for more than 20 min and retains about 90% of its activity after 1 h
4 - 50
1 h, 90% residual activity
40
-
melting temperature
44
-
melting temperature
45
-
5 min, stable
47.5
-
T50 of recombinant wild-type enzyme
48
melting temperature
61.2
melting temperature, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae
61.3
melting temperature, recombinant protein from wild-type Pichia pastoris
63
melting temperature, recombinant protein from wild-type Saccharomyces cerevisiae
64.3
-
T50 of recombinant mutant H91N
65
-
5 min, total inactivation
70
-
10 min, complete inactivation