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1.1.3.7: aryl-alcohol oxidase

This is an abbreviated version!
For detailed information about aryl-alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.7

Reaction

an aromatic primary alcohol
+
O2
=
an aromatic aldehyde
+
H2O2

Synonyms

AAO, AAO2, AAOx, alcohol: O2 oxidoreductase, AOX, arom. alcohol oxidase, aryl alcohol oxidase, arylalcohol oxidase, CpSAO, CtSAO, GaoB, GLRG_02805, GMC oxidoreductase-like protein, HMFO, More, MtGloA, MYCTH_2299749, oxidase, aryl alcohol, salicyl alcohol oxidase, um04044, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.7 aryl-alcohol oxidase

Purification

Purification on EC 1.1.3.7 - aryl-alcohol oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: VAO I and VAO II
-
gel filtration
native enzyme 24.4fold to homogeneity by anion exchange chromatography and gel filtration
-
nickel Sepharose 6 column chromatography
partially by microsome preparation
-
recombinant enzyme
-
recombinant enzyme from Emericella nidulans by gel filtration and anion exchange chromatography
-
recombinant enzyme from Escherichia coli
recombinant enzyme, expressed and secreted from Coprinopsis cinerea strain FA2222, 72fold from culture supernatant by cross-flow filtration, anion exchange chromatography, and two different steps of gel filtration
recombinant FLAG1-tagged enzyme in Escherichia coli strain W3110 solubilized from inclusion bodies 9.6fold by anion exchange chromatography
recombinant mature enzyme from Escherichia coli by anion exchange chromatography
recombinant protein
recombinant wild-type and mutant emzymes from Escherichia coli to homogeneity
three-phase partitioning with 30% (w/v) of ammonium sulfate and t-butanol (1:1 ratio)
-