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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

pH Stability

pH Stability on EC 1.1.3.6 - cholesterol oxidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 2
purified recombinant enzyme, 30°C, 10% activity remaining after 30 min
742934
11
purified recombinant enzyme, 30°C, 30 min, 70% activity remaining
742934
3 - 11
4 - 10
4 - 11
4 - 12.5
Streptoverticillium cholesterolieum
-
22°C, 1 h
710959
4 - 8
25°C, 20 h
710959
4.5 - 10
stable
763627
4.5 - 10.5
ChoRII is stable. Shows higher stability than ChoRI even at pH 11 to 12
763627
5 - 10
-
37°C, 30 min, stable
287646
5 - 6.5
-
60°C, 60 min, stable
656316
5 - 7
-
stable at
684242
5 - 7.5
-
60°C, 1 h
710959
5 - 8
5 - 8.5
-
60 min, more than 80% of the CHO-U activity remains
656321
5 - 9
Corynebacterium cholesterolicum
-
30°C, 1 h
710959
5.5 - 10
purified recombinant enzyme, 30°C, 30 min, stable at
742934
5.5 - 9
-
good pH stability over the pH range of 5.0-9.0
763712
6 - 10
6 - 8.5
-
purified enzyme, completely stable at
743737
7.5
-
60°C, 60 min, about 10% loss of activity
656316
9
-
37°C, 3 h, enzyme retains about 75% of its initial activity
763212
9.5
-
purified enzyme, loss of 85% activity
743737