1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
-
1.1.3.6
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biosensors
-
electrode
-
esterase
-
electrochemical
-
brevibacterium
-
fabric
-
oxidases
-
amperometric
-
film
-
rhodococcus
-
raft
-
cholestenone
-
voltammetry
-
cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
-
erythropolis
-
filipin
-
4-cholesten-3-one
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nocardia
-
medicine
-
3hcholesterol
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electropolymerization
-
lavendulae
-
biotechnology
-
agriculture
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co-immobilized
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methyl-beta-cyclodextrin
-
diagnostics
-
bioelectrode
-
luminol
-
screen-printed
-
electrocatalytic
-
analysis
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3.1.1.13
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polypyrrole
-
multiwalled
-
beta-ol
-
synthesis
-
drug development
- 1.1.3.6
-
biosensors
-
electrode
- esterase
-
electrochemical
- brevibacterium
-
fabric
- oxidases
-
amperometric
-
film
- rhodococcus
-
raft
- cholestenone
-
voltammetry
- cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
-
3hcholesterol
-
electropolymerization
- lavendulae
- biotechnology
- agriculture
-
co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
-
bioelectrode
- luminol
-
screen-printed
-
electrocatalytic
- analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
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Turnover Number
Turnover Number on EC 1.1.3.6 - cholesterol oxidase
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7
epiandrosterone
-
H2O2 production assayed, 100 mM phosphate buffer
0.8
-
assay relies on detection of H2O2 formation
0.8
3beta-hydroxy-5-androsten-17-one
-
H2O2 production assayed, 100 mM phosphate buffer
0.8
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
7
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
8.2
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
8.2
3beta-hydroxy-androst-5-en-17-one
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
28
-
H69A mutant enzyme, 5-cholesten-3-one isomerization, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
250
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
278
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
330
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
332
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
670
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 50 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
9.86
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
13.25
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
16.06
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
37
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
40
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.035
cholesterol
apparent value, mutant enzyme N485L, pH 5.1, temperature not specified in the publication
0.044
cholesterol
apparent value, mutant enzyme N485L, pH 7.0, temperature not specified in the publication
0.073
cholesterol
mutant enzyme N485D, determined by cholest-4-en-3-one detection
0.073
cholesterol
apparent value, mutant enzyme N485D, pH 7.0, temperature not specified in the publication
0.55
cholesterol
-
H69A mutant enzyme, steady state, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
0.8
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation, decreasing length of C17 chain affects turnover negatively
0.86
cholesterol
mutant enzyme F359W, determined by cholest-4-en-3-one detection
1
cholesterol
mutant enzyme G347N, determined by cholest-4-en-3-one detection
1.4
cholesterol
apparent value, mutant enzyme N485D, pH 5.1, temperature not specified in the publication
1.6
cholesterol
-
H69A mutant enzyme, cholesterol oxidation, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
3
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
6
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
9
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
11
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
11
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
12.29
cholesterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
15.48
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
23
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate
23.02
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
30
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate
32
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
42
cholesterol
wild-type enzyme, determined by cholest-4-en-3-one detection
43
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
46
cholesterol
apparent value, wild type enzyme, pH 5.1, temperature not specified in the publication
46
cholesterol
pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate
47
cholesterol
apparent value, wild type enzyme, pH 7.0, temperature not specified in the publication
48
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48.1
cholesterol
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
56
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
57
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
57
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate
60
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate
63
cholesterol
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
63
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
67
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
67
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
67
cholesterol
pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate
68
cholesterol
pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate
75
cholesterol
pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate
89
cholesterol
pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate
105
cholesterol
-
50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol
345
cholesterol
-
50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol
6.82
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
9.56
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
13.68
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
11.78
pregnenolone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
13.71
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
15.25
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
21
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
21
pregnenolone
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
21
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
21
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
24
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
24
pregnenolone
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
24
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
35
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.08
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
1.58
stigmasterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
4.23
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.8
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
7
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
6
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
8.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5