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1.1.3.41: alditol oxidase

This is an abbreviated version!
For detailed information about alditol oxidase, go to the full flat file.

Word Map on EC 1.1.3.41

Reaction

an alditol
+
O2
=
an aldose
 +
H2O2

Synonyms

AldO, HotAldO

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.41 alditol oxidase

Crystallization

Crystallization on EC 1.1.3.41 - alditol oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native wild-type enzyme, hanging-drop vapor diffusion method at 4°C by mixing equal volumes of 14 mg/mL AldO solution in 50 mM potassium phosphate buffer, pH 7.5, with reservoir solutions containing 0.1 M MES/HCl, pH 6.5, 0.2 M MgCl2 and 18-20% w/v PEG4000, 3-4 days, substrate incorporation by soaking the wild-type AldO crystals in a solution consisting of 0.1 M MES/HCl, pH 6.5, 0.2 M MgCl2, 25% w/v PEG 4000, 17.5% sucrose, and 25 mM substrate for 3 h, X-ray diffraction structure determination and analysis at 1.1-1.9 A resolution