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1.1.3.41: alditol oxidase

This is an abbreviated version!
For detailed information about alditol oxidase, go to the full flat file.

Word Map on EC 1.1.3.41

Reaction

an alditol
+
O2
=
an aldose
+
H2O2

Synonyms

AldO, HotAldO

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.41 alditol oxidase

Cloned

Cloned on EC 1.1.3.41 - alditol oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061
expressed in Escherichia coli C41 (DE3) cells
-
expressed in Escherichia coli Top10 cells
-
expression in Escherichia coli
functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm
gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant enzyme expression in the periplasm or on the cell surface of Escherichia coli cells of different strains. The enzyme is differently tagged, i.e. expressed as Tat-AldO or INP-AldO, and exported to the periplasm or to the cell surface of the transformed cells, overview. AldO is successfully displayed at the surface of Escherichia coli using a truncated INP variant, it contains covalently bound FAD, thus has attained a correctly folded and active conformation
-