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1.1.3.4: glucose oxidase

This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.

Word Map on EC 1.1.3.4

Reaction

beta-D-glucose
+
O2
=
D-glucono-1,5-lactone
 +
H2O2

Synonyms

AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.4 glucose oxidase

Storage Stability

Storage Stability on EC 1.1.3.4 - glucose oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C crystalline suspension, stable for at least 8 years
-
-20°C, lyophilised enzyme, minimum of 6 months, remains stable
-
-20°C, purified enzyme, 6 months without addition of additives, complete stable, no loss of activity
-
-20°C, purified thioaniline-functionalized enzyme, at least three months, no noticeable degradation in its activity
-
0°C, as a solid, stable for at least 2 years
3°C, purified enzyme, several years
-
4-5°C, immobilized, 90% activity retained, 8 months
-
4°C, immobilized, 11% loss of activity, 1 year
-
4°C, immobilized, 3 months
-
4°C, immobilized, membrane in dried state, more than 12 months
-
4°C, lyophilized enzyme, no loss of activity after 1 year, 10% loss of activity after 2 years
-
4°C, more than 90% activity retained, 100 mM phosphate buffer, pH 7, immobilized, 4 months
-
4°C, purified recombinant enzyme in 100 mM sodium phosphate, pH 5.1, several months, no loss of activity
4°C, storage for 30 days with a retainment of 90% activity
50°C, purified recombinant immobilized GOx, up to 80% of initial activity is retained after 44 h 50°C, purified recombinant free GOx, loss of 90% of initial activity within 44 h, a further 120 h storage period at 9°C leads to partial denaturation and is not reversible
frozen, at least 1 week
-
lyophilized, over P2O5
-
native GOx retains 43% of its bioactivity after 10 days and completely loses activity within 30 days. The bioconjugates of GOx, i.e. GOx-PPEG-A, GOx-PAA, GOx-PMA and GOx-PTBA, still retain bioactivity of 57%, 53%, 59%, and 68%, respectively after 10 days. 27%, 26%, 39%, and 37% of their origin bioactivity are still maintained after 30 days, respectively. Their bioactivities are lost completely after 45 days, 50 days, 55 days, and 55 days for GOx-PPEG-A, GOx-PAA, and GOx-PMA and GOx-PTBA, respectively
-
reaction rate of free GOx in solution remained stable up to 50 days for all investigated pH values, followed by a dramatic decrease owing to enzyme deactivation
-
storage for 24 h at high temperatures of 50°C and 75°C causes a reduction of activity
-
the lyophilized purified enzyme is stable at -20 °C for a minimum of 6 months.
-