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1.1.3.17: choline oxidase

This is an abbreviated version!
For detailed information about choline oxidase, go to the full flat file.

Word Map on EC 1.1.3.17

Reaction

Betaine aldehyde
+
O2
 +
H2O
=
betaine
 +
H2O2

Synonyms

alkaliphilic choline oxidase, ANI01nite_22550, An_CodA, APChO-syn, CHO, choline oxidase, choline-oxygen 1-oxidoreductase, choline:oxygen 1-reductase, ChOx, ChOx protein, codA, COX

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.17 choline oxidase

Crystallization

Crystallization on EC 1.1.3.17 - choline oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
active site of wild-type choline oxidase, resolution of 1.86 A
anaylsis of biophysical properties of choline oxidase
crystal structure determined by synchrotron X-ray radiation, refined to a resolution of 1.86 A, data collected at 100 K
hanging drop vapor diffusion method, 1.2-1.8 M ammonium sulfate and 10% (v/v) DMSO in 0.1 M Bis-Tris propane (pH 8.5), flash-frozen in liquid nitrogen. Analysis of the flavin C4a-oxygen adduct in choline oxidase
in complex with glycine betaine, hanging drop vapor diffusion method, using 0.1 M magnesium acetate pH 6.0, 50 mM calcium chloride, 2.5% (v/v) glycerol, 10% (w/v) PEG 6000
mutant enzyme S101A, hanging drop vapor diffusion method, using 80 mM sodium cacodylate, 20% (v/v) PEG6000, 20% (v/v) glycerol, 150 mM Mg-acetate at pH 6.0
purified recombinnat wild-type enzyme and mutant V464A, hanging drop vapor diffusion, room temperature, from 10-15% v/v PEG 6000, 50-200 mM magnesium acetate, 200 mM trimethylamine, and 0.08 M sodium cacodylate, pH 6.0, X-ray diffraction structure determination and analysis at 2.2 A resolution