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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
 +
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

Cloned

Cloned on EC 1.1.3.10 - pyranose oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
expressed heterologously in Escherichia coli DH5alpha and BL21(DE3)
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21 Star DE3 cells
expressed in Escherichia coli BL21-DE3, recombinant host strains shown to be different in formation of inclusion bodies at higher growth temperatures, plasmids pSE33 and pRQ5 used for site-directed recombination
expressed in Escherichia coli BL21-DE3, recombinant host strains shown to be different in formation of inclusion bodies between inserts of Trametes ochracea and Trametes pubescens at higher growth temperatures, plasmids pSE33 and pRQ5 used for site-directed recombination
expressed in Escherichia coli BL21-DE3, recombinant protein, expression vector pET21
-
expressed in Escherichia coli BL21-DE3, recombinant protein, plasmids pHL2e and pCL22 encoding the E542K mutant used
expressed in Escherichia coli Rosetta 2(DE3) cells
expression in Escherichia coli
expression in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically active P2OxB with a volumetric yield of 500 units/ml
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
heterologously expressed in E. coli BL21 Star DE3
heterologously expressed in Escherichia coli BL21 (DE3)
heterologously expressed in Escherichia coli BL21 Star DE3
-
heterologously expressed in Escherichia coli DH5 alpha as His-tagged protein
mutant enzymes are expressed in Escherichia coli XL1-blue cells
recombinant expression in Escherichia coli
-
recombinant expression of pyranose 2-oxidase in Escherichia coli strain Rosetta(DE3)pLysS
-
the preproenzyme and the mature enzyme expressed in Escherichia coli
-