1.1.1.B65: (R)-mandelate dehydrogenase [NAD(P)+]

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For detailed information about (R)-mandelate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

Synonyms

RMDH

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.B65 (R)-mandelate dehydrogenase [NAD(P)+]

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Results	in table
2	Activating Compound
4	Cofactor
10	Inhibitors
3	KM Value [mM]
3	Metals/Ions
2	Natural Substrates/ Products (Substrates)
2	Organism
1	pH Optimum
1	pH Range
1	pH Stability
1	Purification (Commentary)
2	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
4	Substrates and Products (Substrate)
2	Subunits
2	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
3	Turnover Number [1/s]

pH Stability

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pH Stability on EC 1.1.1.B65 - (R)-mandelate dehydrogenase [NAD(P)+]

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6 - 9

Pseudomonas putida

purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5

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